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F0F1-ATPase Molecular Motor Biosensor for Rapid Detection of Hepatitis A Visus in Foods

ZHANG Jie,WANG Xiao-jin,XU Mei-ling,ZHANG Hui-yuan,ZHANG Lei,LIU Yan,GU De-zhou,WANG Pei-rong,YUE Jia-chang,CHEN Guang-quan   

  1. 1. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China;2. Huaian Entry-Exit Inspection and Quarantine Bureau, Huai’an 223001, China;3. Linyi Entry-Exit Inspection and Quarantine Bureau, Linyi 276034, China;4. Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
  • Online:2013-08-25 Published:2013-09-03


An F0F1-ATPase molecular motor biosensor was established for rapid detection of Hepatitis A Virus (HAV) in
foods. The biosensor was fabricated by connecting HIV specific probe to the ε subunit of F0F1-ATPase using an avidinbiotin
system. The detection of HAV in samples was based on the difference in fluorescence intensity 10 min after initiation
of ATP synthesis from HAV positive serum and negative control RNA when separately bound to the biosensor. The results
demonstrated that the F0F1-ATPase biosensor exhibited good specificity and high sensitivity to HAV in foods. The sensitivity
of the method was 0.01 ng/mL. The results obtained by this method agreed with those by reverse transcription PCR. The
process of HAV detection with the biosensor can be completed within 1 h. In conclusion, this study has developed a method
for the investigation and detection of HAV contamination in foods.

Key words: biosensor, molecular motor, hepatitis A visus (HAV), rapid detection