Cloning and Expression of a Novel Water Stress Protein from Nostoc commune Vauch. and Its Inhibitory Effect on the Proliferation of Human Colon Cancer SW480 Cells

doi:10.7506/spkx1002-6630-201413029

Abstract

Abstract:

Water stress protein (WSP) gene was amplified by polymerase chain reaction (PCR) from the Nostoc commoune
Vauch. genome (Genbank NO. KF003026), and cloned into the prokaryotic expression vector pET28a. After being
sequenced, the recombinant plasmid pET28a-WSP was transformed into E.coli BL21, and recombinant water stress protein 1
(Re-WSP1) was induced to express by isopropyl-β-D-thiogalactoside (IPTG). The soluble 6 × histidine (His)-tagged fusion
Re-WSP1 was further purified by Ni+-NTA affinity chromatography. The molecular weight of the fused Re-WPS1 was 40 kD
as expected. When applied to cells in culture, Re-WSP1 could significantly suppress the growth of SW480 colon cancer
cells, but had a negligible effect on that of FHC normal colon epithelial cells. In addition, Re-WSP1-treated SW480 cells
displayed the apoptotic characteristics of chromatin condensation and typical fragmented nuclei, as demonstrated by DAPI
staining. Re-WSP1 could also activate procaspase-8 and procaspase-3, resulting in their cleavage into active fragments.
Taken together, Re-WSP1 has shown obvious anti-colon cancer effects on SW480 cells, and induced apoptosis by caspasedependent
pathway in SW480 cells.

Key words: Nostoc commune Vauch., recombinant water stress protein 1 (Re-WSP1), expression and purification, colon cancer, cell apoptosis

GUO Song-jia, SHAN Shu-hua, JIN Xiao-ting, LI Zong-wei, SONG Li, LI Zhuo-yu. Cloning and Expression of a Novel Water Stress Protein from Nostoc commune Vauch. and Its Inhibitory Effect on the Proliferation of Human Colon Cancer SW480 Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201413029.

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Cloning and Expression of a Novel Water Stress Protein from Nostoc commune Vauch. and Its Inhibitory Effect on the Proliferation of Human Colon Cancer SW480 Cells

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