Intervention effects of Lycium barbarum juice on the metabolism of DEHP based on the UGT1 detoxification pathway

Abstract

Abstract: Purpose: This study aimed to explore the intervention effects of Lycium barbarum juice (LBJ) on the metabolism of di-(2-ethylhexyl) phthalate (DEHP); through enzyme activity analysis of the UDP glucuronosyltransferase1 (UGT1) in hepatic and metabolic detoxification profile of rats exposed to DEHP. Methods: Sixty Sprague Dawley male rats were randomly divided into three groups (n = 20 each): control group, DEHP group and LBJ group. DEHP and LBJ group were administered normal saline and LBJ respectively by gavage once daily for 7 days after receiving a single dose of 3000 mg/kg·bw DEHP dissolved in sesame oil. The control group was treated with normal saline for 7 day after receiving sesame oil of the same volume. The rat urine samples were collected during 24 hours on 7 consecutive days. 5 rats were sacrificed randomly in each group after 1 day, 3, 5, 7 days of exposure. The activity of UGT1 was determined by ELISA kit while the contents of mono-(2-ethylhexyl) phthalate (MEHP) in serum and urine and phthalic acid (PA) in urine were detected by HPLC. Results: The activity of UGT1 of LBJ group was significantly increased on the 5th day compared to DEHP and control groups (P<0.05). The content of serum MEHP in LBJ group decreased significantly, whereas the contents of urine MEHP and PA increased compared to DEHP group. Conclusions: LBJ could promote the metabolism and excretion of DEHP through improving the activity of UGT1, which made it hopeful to become a health care product or functional food against the potential hazard of phthalate esters (PAEs) or other poisonous chemicals.

Key words: di-(2-ethylhexyl) phthalate (DEHP), the UDP glucuronosyltransferase (UGT), UGT1, Lycium barbarum juice (LBJ), intervention

CLC Number:

References

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