Abstract: Objective: To explore the effect of probiotic intervention frequency and cycle on blood lipids in hyperlipidemic rats. Methods: A rat model of hyperlipidemia was established through feeding a high fat diet for 4 consecutive weeks, and two intervention groups were designed by using the probiotic Lactobacillus plantarum (Lp) 67 combined with intermittent fasting, a dietary strategy for treating obese people: intragastric administration with an Lp 67 suspension of 108 CFU/mL 5 times weekly plus physiological saline for the remaining 2 days, and administration with the same suspension every other day weekly plus physiological saline for the remaining days. Body mass was weighed during the intervention period and visceral organ indexes after 4, 6, and 8 weeks of intervention and the serum levels of total cholesterol, total triglyceride, high-density liptein cholesterol and low-density liptein cholesterol were measured. The two intervention groups were compared with the 7-day continuous intervention, control and model groups in terms of all these parameters. Results: Compared with the model group, all three intervention treatments could effectively control blood lipids in rats, and there were significant differences among these intervention groups (P＜0.05). In general, Lp 67 intervention 5 times a week for 6 consecutive weeks was the most effective in improving the blood lipid profile. Conclusion: The frequency and cycle of probiotic intervention have significant effects on improving blood lipids in hyperlipidemic rats.

Key words: probiotics, frequency of intervention, intervention cycle, hyperlipidemia, hypolipidemic