食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 50-53.doi: 10.7506/spkx1002-6300-200918006

• 工艺技术 • 上一篇    下一篇

玉米花丝多糖脱色方法的研究

何余堂,宫照杰   

  1. 渤海大学生物与食品科学学院,辽宁省食品质量安全与功能性食品重点实验室
  • 收稿日期:2009-05-24 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 何余堂 E-mail:heyutang@163.com
  • 基金资助:

    辽宁省教育厅高校重点实验计划资助项目(2008S003)

Comparative Study of Decolorization of Crude Corn Silk Polysaccharide

HE Yu-tang,GONG Zhao-jie   

  1. Liaoning Provincial Key Laboratory of Food Quality Safety and Functional Food, College of Biotechnology and Food Science,
    Bohai University, Jinzhou 121000, China
  • Received:2009-05-24 Online:2009-09-15 Published:2010-12-29
  • Contact: HE Yu-tang E-mail:heyutang@163.com

摘要:

以玉米花丝为原料分离活性多糖,并对多糖的脱色方法进行研究。分别采用活性碳、次氯酸钠、过氧化氢和大孔阴离子交换树脂D315 对玉米花丝多糖进行脱色,通过对多糖脱色率、保留率及抑菌性的比较发现,四种方法对花丝多糖均有脱色效果。次氯酸钠和双氧水脱色后多糖保留率较低,抑菌性较差。活性炭的脱色率为87.9%,多糖保留率为81.5%。树脂D315 的脱色率为83.4%,多糖保留率为76.6%。活性炭和树脂脱色法明显优于次氯酸钠和过氧化氢脱色法,但活性炭脱色后多糖溶液中残留的活性炭难以清除,对多糖品质有一定影响;树脂脱色是较好的方法。

关键词: 玉米花丝, 活性多糖, 分离, 脱色

Abstract:

Active carbon, sodium hypochlorite, hydrogen peroxide and D315 macroporous anion exchange resin were separately used for the decolorization of crude polysaccharide from corn silks. All four methods displayed decolorization effects on crude corn silk polysaccharide in terms of decolorization efficiency, retention rate of polysaccharide and antibacterial activity. Decolorization using sodium hypochlorite or hydrogen peroxide led to low retention rate of polysaccharide and decrease of antibacterial activity. The decolorization efficiencies by active carbon and macroporous anion exchange resin decolorization methods were 87.9% and 83.4%, and the retention rates of polysaccharide were 81.5% and 76.6%, respectively. Both active carbon and D315 resin exhibited better decolorization capability than sodium hypochlorite and hydrogen peroxide. Nevertheless, active carbon residue in polysaccharide solution was difficult to remove, which had a disadvantage effect on polysaccharide quality. In general, resin decolorization method is the best choice.

Key words: corn silk, active polysaccharide, separation, decolorization

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