食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 152-154.doi: 10.7506/spkx1002-6300-200918031

• 工艺技术 • 上一篇    下一篇

利用超临界技术萃取石榴叶总酚工艺研究

王 华   

  1. 中国农业科学院柑桔研究所
  • 收稿日期:2009-05-21 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 王 华 E-mail:wanghua40@126.com

Orthogonal Array Design as An Approach to Optimize Supercritical Fluid Extraction Conditions of Total Polyphenols from Punica granatum L. Leaves

WANG Hua   

  1. Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, China
  • Received:2009-05-21 Online:2009-09-15 Published:2010-12-29
  • Contact: WANG Hua E-mail:wanghua40@126.com

摘要:

利用超临界CO2 装置对石榴叶中总酚提取工艺进行研究,以期得到高生理活性和高纯度的石榴叶活性物质。通过单因素和正交试验,得到最佳萃取条件为料液比1:2.5(g/ml)、萃取压力25MPa、萃取温度60℃、萃取时间120min。活性物质得率为0.1728%,纯度为42.61%。

关键词: 石榴叶, 总酚, 超临界萃取

Abstract:

Total polyphenols in Punica granatum L. leaves were extracted using supercritical CO2 fluid with the addition of absolute ethanol as a cosolvent. In order to maximize yield of total polyphenols, four crucial extraction parameters such as ratio of material to liquid, and extraction pressure, temperature and time were optimized by single-factor and orthogonal array methods. A maximum yield of total polyphenols at 42.61% purity was obtained by the 120 min extraction at 25 MPa and 60 ℃ with a ratio of material to liquid of 1:2.5.

Key words: leaves of Punica granatum L., total polyphenols, supercritical fluid extraction

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