食品科学 ›› 2009, Vol. 30 ›› Issue (20): 113-117.doi: 10.7506/spkx1002-6300-200920020

• 工艺技术 • 上一篇    下一篇

紫心甘薯多糖提取工艺研究

高秋萍1,阮红2   

  1. 1. 浙江大学生命科学学院
    2. 浙江大学城市学院医学与生命科学学院
  • 收稿日期:2008-10-09 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 阮红 E-mail:ruanhong@zucc.edu.cn
  • 基金资助:

    杭州市科技局重点实验室科技创新项目(20080432T05)

Extraction of Purple Sweet Potato Polysaccharides

GAO Qiu-ping1,RUAN Hong2,*   

  1. 1. College of Life Sciences, Zhejiang University, Hangzhou 310027, China;
    2. School of Medicine and Life Sciences, Zhejiang University City College, Hangzhou 310015, China
  • Received:2008-10-09 Online:2009-10-15 Published:2010-12-29
  • Contact: RUAN Hong2,* E-mail:ruanhong@zucc.edu.cn

摘要:

为了开发利用浙江省临安市太阳镇实验基地的紫心甘薯资源,对紫心甘薯中多糖的提取工艺进行了研究。对影响紫心甘薯多糖提取率的参数(料液比、提取温度、提取时间、提取次数、pH值、醇沉浓度)分别进行了单因素试验,在单因素试验的基础上,对其中3个主要影响因子(料液比、温度、时间) 进行正交试验,进一步比较了几种粗多糖脱蛋白和脱色的方法。最终确定了紫心甘薯多糖最佳提取条件为:料液比1:15、温度70℃、浸提2h、提取2次、pH中性、85%乙醇醇沉、3%三氯乙酸法脱蛋白、硅藻土脱色。

关键词: 紫心甘薯, 水溶性多糖, 提取工艺, 优化

Abstract:

The aim of the present study was to develop an optimal preparation procedure of polysaccharides from purple sweet potato (PPSP) consisting of hot water extraction, alcohol precipitation, deprotein and decolorization. A series of single factor experiments were conducted to investigate effects of extraction temperature, extraction time, pH, solid/liquid ratio, extraction times and concentration of alcohol in aqueous solution (as a precipitant) on PPSP yield. On the basis of this, three main affecting factors, including solid/liquid ratio, extraction time and extraction temperature were optimized for maximizing PPSP yield using a three-level orthogonal array design. Results showed that the optimal preparation procedure of PPSP consisted of twice extraction for 2 h at 70 , pH 7.0 with a solid/liquid ratio of 1:15, precipitation with 85% aqueous alcohol, deprotein with 3% trichloroacetic acid, and decolorization with diatomite.

Key words: purple sweet potato, water-soluble polysaccharides , extraction technology , optimization

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