食品科学 ›› 2009, Vol. 30 ›› Issue (20 ): 265-268.doi: 10.7506/spkx1002-6300-200920056

• 分析检测 • 上一篇    下一篇

安徽河南粮食中脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的污染调查

熊凯华1,胡 威1,汪孟娟1,魏 华1,程波财1,2,*   

  1. 1.南昌大学 食品科学与技术国家重点实验室 2.中南大学资源加工与生物工程学院
  • 收稿日期:2009-06-30 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 程波财1,2,* E-mail:chengbocai@126.com
  • 基金资助:

    国家自然科学基金项目(3086008)

A Survey on Contamination of Deoxynivalenol and Zearalenol in Maize and Wheat from Anhui and Henan Province

XIONG Kai-hua1,HU Wei1,WANG Meng-juan1,WEI Hua1,CHENG Bo-cai1,2,*   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
    2. School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China
  • Received:2009-06-30 Online:2009-10-15 Published:2010-12-29
  • Contact: CHENG Bo-cai1,2,* E-mail:chengbocai@126.com

摘要:

目的:调查安徽、河南两省粮食中镰刀菌毒素污染情况。方法:以玉米、小麦为材料,分别采用酶联免疫吸附法(ELISA)和高效液相色谱法(HPLC)检测了脱氧雪腐镰刀菌烯醇(DON)和玉米赤霉烯酮(ZEN)的含量,采用SPSS软件对检测结果进行统计学分析。结果:安徽河南两省玉米、小麦中的DON和ZEN含量平均值分别为424.0μg/kg和187.2μg/kg,检出率分别为76.7%和75.3%。其他省份的玉米、小麦中DON和ZEN含量的平均值为52.2μg/kg和24.1μg/kg,检出率分别为60%和70%。结论:和其他省份相比,安徽、河南两省的粮食受镰刀菌毒素污染程度更严重。根据现有的国家限量标准,ZEN的超标率比DON更高。

关键词: 镰刀菌毒素, 粮食, 污染水平

Abstract:

Objective: To survey contamination level of Fusarium toxins in maize and wheat from Anhui and Henan province. Methods: Enzyme-linked immunosorbent assay (ELISA) and HPLC were applied for the detection and determination of deoxynivalenol (DON) and zearalenol (ZEN) in maize and wheat respectively. Statistical analysis was performed using SPSS 10.0 software. Results: The average contents of DON and ZEN in maize and corn from Anhui and Henan province were 424.0 and 187.2 μg/kg, and the contamination rates were 76.7% and 75.3%, respectively. In contrast, the average contents of DON and ZEN in maize and wheat from other provinces were 52.2 and 24.1 μg/kg, and the contamination rates were 60% and 70%, respectively. Conclusion: The contamination levels of DON and ZEN in maize and wheat from Anhui and Henan province are much higher than those from other provinces, and ZEN exhibits a higher percentage of exceeding the level specified in the Chinese National Standard compared with DON.

Key words: Fusarium toxins, maize and wheat, contamination level

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