食品科学 ›› 2009, Vol. 30 ›› Issue (20 ): 340-342.doi: 10.7506/spkx1002-6300-200920075

• 分析检测 • 上一篇    下一篇

液质法测定动物源性食品中肾上腺素、多巴胺

牛晋阳,郭文萍,孙 焕,宋永青   

  1. 中国肉类食品综合研究中心
  • 收稿日期:2009-08-25 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 牛晋阳

HPLC-MS-MS Determination of Adrenalin and Dopamine in Animal-derived Foods

NIU Jin-yang,GUO Wen-ping,SUN Huan,SONG Yong-qing   

  1. China Meat Research Center, Beijing 100068, China
  • Received:2009-08-25 Online:2009-10-15 Published:2010-12-29
  • Contact: NIU Jin-yang

摘要:

为同时测定动物源性食品中肾上腺素、多巴胺的含量,建立HPLC-MS-MS检测方法。该两种物质的线性范围均为3~1500μg/kg。低、中、高三种浓度加标回收率均为90%~101%;检出限分别为肾上腺素1μg/kg、多巴胺3μg/kg。本方法快速简便,灵敏度高,选择性好,测定结果令人满意,可在各食品检测机构推广应用。

关键词: 肾上腺素, 多巴胺, 动物源性食品, 液质法

Abstract:

In order to determine adrenalin and dopamine in animal-derived foods, a sensitive and rapid HPLC-MS-MS method was established. The standard curve was linear within the concentration range of 3-1500  μg/kg. The spike recovery rates of adrenalin and dopamine at high, medium and low levels averaged 90%-101%. Meanwhile, the limit of detection was 1 μg/kg for adrenalin and 3 μg/kg for dopamine, respectively. This method is simple, sensitive and highly selective so that it can be extensively applied in food quality control.

Key words: adrenalin, dopamine, animal food, HPLC-MS-MS method

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