食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 63-65.doi: 10.7506/spkx1002-6300-200921015

• 基础研究 • 上一篇    下一篇

金银花提取液抗氧化活性研究

罗 磊,郭晓园   

  1. 河南科技大学食品与生物工程学院
  • 收稿日期:2009-06-25 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 罗 磊, E-mail:Luolei_cn@yahoo.com.cn
  • 基金资助:

    河南省教育厅科技攻关项目(2007550004)

Antioxidant Activity of Honeysuckle Extract

LUO Lei,GUO Xiao-yuan   

  1. Food and Bioengineering Department, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2009-06-25 Online:2009-11-01 Published:2010-12-29
  • Contact: LUO Lei, E-mail:Luolei_cn@yahoo.com.cn

摘要:

采用三价铁离子还原法、羟自由基(·OH)体系和二苯代苦味酰基自由基(DPPH·)体系对金银花提取液和绿原酸标准品抗氧化活性进行研究,以抗坏血酸为对照。结果表明:金银花提取液清除·OH 和DPPH·的能力最强,对·OH 半数抑制浓度(IC50)为0.0273mg/ml,提取液的绿原酸浓度为0.006mg/ml 时对DPPH·的抑制率达80%;而绿原酸标准品的还原能力要大于金银花提取液(2.66 倍)和抗坏血酸(2.42 倍);绿原酸为金银花提取液抗氧化作用的主要物质。

关键词: 金银花, 绿原酸, 抗氧化活性

Abstract:

The antioxidant activities of honeysuckle extract and chlorogenic acid were evaluated by ferric ion reducing antioxidant power (FRAP) assay, hydroxyl free radical array and DPPH free radical assay using vitamin C as the reference. Results showed that honeysuckle extract had strong scavenging capacity for hydroxyl and DPPH free radicals. The IC50 of honeysuckle extract for scavenging hydroxyl free radicals was 0.0273 mg/ml. The scavenging rate of honeysuckle extract for DPPH free radicals was 80% when its chlorogenic acid content was 0.006 mg/ml. Meanwhile, the reducing power of chlorogenic acid standard was 2.66- fold and 2.42-fold stronger than that of honeysuckle extract and vitamin C, respectively. Therefore, chlorogenic acid is the major antioxidant component in honeysuckle extract.

Key words: honeysuckle, chlorogenic acid, antioxidant activity

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