食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 229-233.doi: 10.7506/spkx1002-6300-200921054

• 生物工程 • 上一篇    下一篇

六种蜂花粉中酶活性的研究

张红城1,程 蒙2,董 捷1,*,孙庆申3   

  1. 1.中国农业科学院蜜蜂研究所 2. 华南师范大学 激光生命科学教育部重点实验室 3.黑龙江大学生命科学学院,微生物黑龙江省高校重点实验室
  • 收稿日期:2009-06-21 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 董 捷 E-mail:jiedon@126.com
  • 基金资助:

    国家公益性行业(农业)科研专项经费项目(nyhyzx07-041);中国韩国BRICAAS 和NIAST 合作项目(2008-01)

Enzymological Activity of Extracts from Six Pollens

ZHANG Hong-cheng1,CHENG Meng2,DONG Jie1,*,SUN Qing-shen3   

  1. 1. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China;
    2. Key Laboratory of Laser Life Science, Ministry of Education, South China Normal University, Guangzhou 510631, China;
    3. Heilongjing Provincal Key Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin 150080, China
  • Received:2009-06-21 Online:2009-11-01 Published:2010-12-29
  • Contact: DONG Jie E-mail:jiedon@126.com

摘要:

6 种蜂花粉:杏花、桃花、荷花、油菜、拉拉秧、向日葵均含有多种酶类。实验分别对6 种蜂花粉的过氧化氢酶、过氧化物酶、脂肪氧合酶、超氧化物歧化酶、苯丙氨酸裂解酶这五类酶的活性进行测定,通过其酶活性的高低衡量蜂花粉的新鲜程度。

关键词: 蜂花粉, 酶, 酶活力

Abstract:

Enzyme is a major factor involved in the process of cell metabolism, which also contributes to food preservation. Six kinds of pollens from apricot flower, peach blossom, lotus, rape, lalayang, and sunflower contain a variety of enzymes. The activities of catalase, peroxidase, lipoxygenase, superoxide dismutase and phenylalanine lyase in 0.2 mol/L phosphate buffer extracts from these pollens were determined so that the activity level of these enzymes was used as criteria for evaluating the freshness of pollens.

Key words: pollens, enzyme, enzymatic activity

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