食品科学 ›› 2009, Vol. 30 ›› Issue (21): 241-245.doi: 10.7506/spkx1002-6300-200921057

• 生物工程 • 上一篇    下一篇

一株高效降解胆固醇乳酸菌的筛选鉴定及其益生潜能初探

曾小群1, 潘道东,1.2,杨瑶1,郭宇星1,刘颖婷1   

  1. 1. 南京师范大学 国家乳品加工技术研发分中心
    2. 宁波大学生命科学与生物工程学院
  • 收稿日期:2008-12-31 修回日期:2009-07-29 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 潘道东 E-mail:daodongpan@163.com
  • 基金资助:

    国家“863”计划项目(2007AA10Z357) ;“十一五”国际科技支撑计划项目(2006BAD04A12)

A High Cholesterol-reducing Lactic Acid Bacterial Strain: Screening, Identification and Probiotic Potential Evaluation

ZENG Xiao-qun1 PAN Dao-dong1,2,* YANG Yao1 GUO Yu-xing1 LIU Ying-ting1   

  1. 1.Branch Center of National Dairy Products Processing Technology Development, Nanjing Normal University, Nanjing 210097,
    China 2.College of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China
  • Received:2008-12-31 Revised:2009-07-29 Online:2009-11-01 Published:2010-12-29
  • Contact: PAN Dao-dong E-mail:daodongpan@163.com

摘要:

从新鲜牛粪中分离出的5株乳酸菌中筛选得到一株具有高效降胆固醇能力的乳酸菌,其体外胆固醇脱出率达到50.83%。经API50CHL系统及16S rRNA序列比对鉴定为发酵乳杆菌Lactobacillus fermentum,命名为发酵乳杆菌DF-4。模拟胃液中的低pH和小肠中的胆汁盐浓度进行了耐受性试验,结果表明该菌株具有较强的耐酸和耐胆盐的性能,对致病大肠杆菌和金黄色葡萄球菌均有显著抑制作用,是一株具有开发前景的高效降胆固醇的益生菌。

关键词: 筛选, 鉴定, 发酵乳杆菌DF-4, 降胆固醇, 益生菌

Abstract:

A probiotic strain with an excellent cholesterol-reducing ability of 50.83 % was screened from 5 strains of lactic acid bacteria, which were isolated from fresh cattle feces. This strain was identified as Lactobacillus fermentum with API identification system and 16srRNA sequence alignment and was nominated as Lactobacillus fermentum DF-4. It exhibited a strong tolerance to 0.3% bile salt and low pH and an antagonistic effect against both enteropathogenic E.coli and S.aureas when incubated in the simulated gastroenteric environment. These results indicate that Lactobacillus fermentum DF-4 is a potential probiotics with an excellent cholesterol-reducing effect.

Key words: screening, identification, Lactobacillus fermentum DF-4 , cholesterol-reducing effect , probiotics

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