食品科学 ›› 2010, Vol. 31 ›› Issue (3): 182-184.doi: 10.7506/spkx1002-6300-201003041

• 生物工程 • 上一篇    下一篇

溶氧对假单胞菌TS1138生产L-半胱氨酸合成酶系的影响

怀丽华1,陈 宁2   

  1. 1.天津商业大学商学院 2.天津科技大学生物工程学院
  • 收稿日期:2009-04-16 修回日期:2009-09-28 出版日期:2010-02-01 发布日期:2010-12-29
  • 通讯作者: 怀丽华 E-mail:hlh55@163.com
  • 基金资助:

    天津市科委应用基础研究重点基金项目(05YFJZJC00901)

Effect of Dissolved Oxygen on Production of L-cysteine Synthetase by Pseudomonas sp. TS1138

HUAI Li-hua1 CHEN Ning2   

  1. 1. School of Business, Tianjin University of Commerce, Tianjin 300134, China
    2. College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China
  • Received:2009-04-16 Revised:2009-09-28 Online:2010-02-01 Published:2010-12-29
  • Contact: HUAI Li-hua E-mail:hlh55@163.com

摘要:

假单胞菌(Pseudomonas sp.)TS1138在含有DL-2-氨基-Δ2-噻唑啉-4-羧酸(DL-2-amino-Δ2-thiazoline-4-carboxylic acid,DL-ATC)的环境中能够转化DL-ATC生成L-半胱氨酸的酶系。通过固定不同溶氧浓度,探索溶氧对假单胞菌TS1138发酵产酶的影响。摇瓶实验结果表明:溶氧浓度过低,菌体生长缓慢,细胞产酶能力较低;溶氧浓度过高,菌体生长速度快,但不利于细胞产酶。7L罐发酵实验结果表明:在产酶中后期,应根据溶氧浓度变化来调节搅拌转速或空气流量,使溶氧浓度控制在30%以上。

关键词: L-半胱氨酸, 溶氧, 假单胞菌

Abstract:

Pseudomonas sp. TS1138 has potential to produce L-cysteine synthetase through asymmetrical hydrolysis of DL-2-amino-Δ2-thiazoline-4-carboxylic acid (DL-ATC). The effect of dissolved oxygen level on the production of L-cysteine synthetase was investigated in shake flasks or 7 L bioreactor. Results indicated that the cell growth and the production of L-cysteine synthetase were inhibited at low dissolved oxygen level. Although cell growth was improved at the high dissolved oxygen level, the inhibition against production of L-cysteine synthetase was still observed in shake flasks. In 7 L bioreactor, dissolved oxygen concentration controlled at more than 30% was helpful for improving the cell growth and the production of L-cysteine synthetase through regulating agitation rate and air flow rate during the middle and late stage.

Key words: L-cysteine, dissolved oxygen, Pseudomonas sp.

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