关键词: 大蒜精油；莓实假单胞菌；猪肉；抑菌机制

Abstract: In order to explore the pork spoilage potential of Pseudomonas fragi and the antibacterial mechanism of garlic essential oil, P. fragi was isolated from chilled pork under aerobic storage conditions and tested its spoilage capacity. The isolates with strong spoilage capacity were selected for further investigation. The antibacterial activity of garlic essential oil against the selected strains was evaluated by determining the minimum inhibitory concentration (MIC) and plotting the bacterial growth curve. Furthermore, the antibacterial mechanism was investigated by scanning electron microscopic (SEM) observation as well as measuring cell membrane permeability, bacterial motility and metabolic activity. The results showed that P. fragi could cause pork spoilage. Garlic essential oil exhibited a strong inhibitory effect against P. fragi derived from pork, with a MIC of 0.125 μL/mL. Garlic essential oil could disrupt the normal structural morphology of P. fragi, enhance the permeability of the cell membrane, and inhibit bacterial metabolic activity and mobility. The results from this study can provide a theoretical foundation for the application of garlic essential oil as a natural food preservative.

Key words: garlic essential oil; Pseudomonas fragi; pork; antibacterial mechanism