食品科学 ›› 2010, Vol. 31 ›› Issue (4): 123-126.doi: 10.7506/spkx1002-6300-201004028

• 工艺技术 • 上一篇    下一篇

应用膜过滤提取灵芝多糖的研究

毛健1.2,马海乐1   

  1. 1.江苏大学食品与生物工程学院 2.江南大学食品学院
  • 收稿日期:2009-11-02 出版日期:2010-02-15 发布日期:2010-12-29
  • 通讯作者: 毛健 E-mail:biomao@263.net;jzw640@163.com
  • 基金资助:

    国家“863”计划项目(2007AA10Z346)

Membrane Filtration for Separation of Polysaccharides from Fermentation Broth of Ganoderma lucidum

MAO Jian1,2,MA Hai-le1,*   

  1. 1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
    2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Received:2009-11-02 Online:2010-02-15 Published:2010-12-29
  • Contact: MAO Jian E-mail:biomao@263.net;jzw640@163.com

摘要:

从工业化的角度将超滤技术应用到灵芝多糖的提取过程中,并通过AcA44 凝胶层析的方法对将发酵原液、膜过滤后的截留液及透过液进行AcA44 层析分析。结果表明:将灵芝多糖发酵上清液运用膜过滤,不仅可去除部分小分子质量的多糖,提高大分子质量多糖的比例,而且使得后酒精沉淀获得的产品品质明显得到提高。

关键词: 膜过滤, 灵芝多糖, AcaA44凝胶层析

Abstract:

Ultrafiltration was used for the separation of polysaccharides from the fermentation broth of Ganoderma lucidum to simulate industrial production. Meanwhile, the molecular weight distribution of the fermentation broth and its filtrate and retentate was investigated by AcA44 gel chromatography. Results showed that ultrafilitration resulted in the removal of parts of small molecule weight polysaccharides, the increase of proportion of big molecule weight ones and the obvious improvement of quality of products resulting from the following ethanol precipitation.

Key words: membrane filtration, Ganoderma lucidum polysaccharide, AcaA44 gel chromatography

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