黑灵芝多糖对小鼠结肠癌细胞内cAMP/PKA信号通路的作用

doi:10.7506/spkx1002-6630-201323055

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 271-275.doi: 10.7506/spkx1002-6630-201323055

黑灵芝多糖对小鼠结肠癌细胞内cAMP/PKA信号通路的作用

张莘莘，聂少平，谢明勇*

南昌大学食品科学与技术国家重点实验室，江西南昌 330047

收稿日期:2013-07-05 修回日期:2013-11-05 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 谢明勇 E-mail:myxie@ncu.edu.cn;xmync@sina.com.cn
基金资助:
国家自然科学基金项目(31071532；31130041)；“十二五”国家科技支撑计划项目(2012BAD33B06)；
食品科学与技术国家重点实验室目标导向项目(SKLF-MB-201001)

Effect of Ganoderma atrum Polysaccharide on cAMP/PKA Pathway in Mouse Colon Cancer Cells

ZHANG Shen-shen，NIE Shao-ping，XIE Ming-yong*

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2013-07-05 Revised:2013-11-05 Online:2013-12-15 Published:2014-01-03
Contact: XIE Ming-yong E-mail:myxie@ncu.edu.cn;xmync@sina.com.cn

摘要/Abstract

摘要：

研究黑灵芝多糖(PSG-1)对结肠癌细胞内环磷酸腺苷(cAMP)/蛋白激酶A(PKA)信号途径的影响。实验设5组：空白对照组，50、100、200μg/mL PSG-1组，5-氟尿嘧啶(5-Fu)阳性对照组。采用流式细胞术检测细胞的凋亡，用IBL试剂盒检测胞内cAMP的浓度、PKA的活性，半定量RT-PCR法分析cAMP和PKA的mRNA表达变化。结果表明：PSG-1能诱导CT26细胞凋亡；PSG-1显著提高细胞内cAMP的浓度，3种质量浓度PSG-1作用后cAMP浓度分别比空白对照组升高了20.18%、38.67%和56.25%(P＜0.05)；与空白对照组相比，PSG-1各组细胞的PKA活性分别上升了15.42%、18.76%和38.26%(P＜0.05)。另外，PSG-1组胞内的PKA和CREB mRNA的表达量比空白对照组也明显增加。结果显示，PSG-1能够引起胞内cAMP 浓度升高、PKA活性增强，从而激活小鼠结肠癌细胞内cAMP/PKA信号途径。

关键词: 黑灵芝多糖, CT26细胞, cAMP/PKA通路

Abstract:

Ganoderma atrum polysaccharide-1 (PSG-1) has been reported to be natural chemopreventive in several types
of tumor cells. In our previous study, we found that PSG-1 could significantly inhibit the tumor growth. We hypothesized
that PSG-1 might exert its anticancer effect by activating cAMP/protein kinase A (PKA) pathway. The present study was
designed to analyze the effect of PSG-1 on the cAMP/PKA pathway in mouse colon cancer CT26 cells. The cells were
treated with 0, 50, 100 μg/mL, and 200 μg/mL PSG-1 or 20 μg/mL 5-fluorouracil, respectively, for 48 h. The ratio of
apoptotic CT26 cells was measured by flow cytometry analysis. The cellular cAMP content was tested using a cAMP assay
kit. The activity of PKA was measured using a PKA activity assay kit. Reverse transcript-polymerase chain reaction (RT-PCR)
was used to analyze the mRNA expressions of PKA and cAMP response element binding protein (CREB) of the cells. The
results showed that PSG-1 significantly induced the apoptosis of the CT26 cells and that cAMP concentration in the cells
treated with PSG-1 was dramatically increased by 20.18%, 38.67% and 56.25% (P ＜ 0.05), respectively. The PKA activity
was increased by 15.42%, 18.76% and 38.26%, respectively. The mRNA levels of both PKA and CREB of the cells treated
with PSG-1 were increased. The current study suggests that PSG-1 activates the cAMP/PKA pathway in colon cancer cells
by inducing cAMP production and activating PKA protein.

Key words: Ganoderma atrum polysaccharide, CT26 cells , cAMP/PKA pathway

中图分类号:

Q949.32

张莘莘，聂少平，谢明勇*. 黑灵芝多糖对小鼠结肠癌细胞内cAMP/PKA信号通路的作用[J]. 食品科学, 2013, 34(23): 271-275.

ZHANG Shen-shen，NIE Shao-ping，XIE Ming-yong*. Effect of Ganoderma atrum Polysaccharide on cAMP/PKA Pathway in Mouse Colon Cancer Cells[J]. FOOD SCIENCE, 2013, 34(23): 271-275.

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黑灵芝多糖对小鼠结肠癌细胞内cAMP/PKA信号通路的作用

Effect of Ganoderma atrum Polysaccharide on cAMP/PKA Pathway in Mouse Colon Cancer Cells

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