食品科学 ›› 2010, Vol. 31 ›› Issue (5): 23-25.doi: 10.7506/spkx1002-6300-201005006

• 基础研究 • 上一篇    下一篇

蛇菰提取物体外抗氧化活性研究

邓 靖1,2,莫正昌1,2,汲广全1,2,杨 娟2,*   

  1. 1.贵州大学生命科学学院 2.贵州省中国科学院天然产物化学重点实验室
  • 收稿日期:2009-06-09 出版日期:2010-03-01 发布日期:2010-12-29
  • 通讯作者: 杨娟 E-mail:yangxz2002@126.com;linyj401@tom.com
  • 基金资助:

    国家自然科学基金项目(30760297);国家重点基础研究发展计划项目(2009CB526512)

Antioxidant Activity of Extract from Balanophora spicata Hayata in vitro

DENG Jing1,2,MO Zheng-chang1,2,JI Guang-quan1,2,YANG Juan2,*   

  1. 1. College of Life Science, Guizhou University, Guiyang 550025, China;2. The Key Laboratory of Chemistry for Natural
    Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China
  • Received:2009-06-09 Online:2010-03-01 Published:2010-12-29
  • Contact: YANG Juan2,* E-mail:yangxz2002@126.com;linyj401@tom.com

摘要:

采用DPPH·法及Fe3+ 还原力法对蛇菰提取物的抗氧化活性进行研究,分别测定蛇菰乙醇提物、石油醚提取物、氯仿提取物、乙酸乙酯提取物、正丁醇提取物、水层部分、水提粗多糖及碱提粗多糖的抗氧化作用,同时以VC 作为阳性对照。结果表明:蛇菰提取物具有较强的抗氧化活性,尤其是乙酸乙酯提取物、正丁醇提取物、总醇提取物及水提粗多糖;它们对DPPH·的清除作用与VC 相当,其中乙酸乙酯提取物IC50 值为6.0μg/mL,活性略高于VC,同时以上提取物对Fe3+ 也具有很强的还原能力。

关键词: 蛇菰, 提取物, 抗氧化活性, DPPH·清除率, 还原力

Abstract:

DPPH· free radical scavenging assay and ferric reducing power assay in vitro were used to evaluate the antioxidant activities of ethanol extract, petroleum ether extract, chloroform extract, ethyl acetate extract, n-butanol extract, water extraction fraction, water-soluble crude polysaccharides and alkaline-soluble crude polysaccharides from Balanophora spicata Hayata. Results indicated that the extracts from Balanophora spicata Hayata all exhibited strong scavenging capacity against DPPH free radicals, especially ethyl acetate extract, n-butanol extract, ethanol extract and water-soluble crude polysaccharides, which were nearly equivalent to vitamin C for DPPH free radical scavenging activity. The fact that ethyl acetate extract exhibited an IC50 value of 6.0 μg/mL suggested that a little stronger antioxidant activity of ethyl acetate extract than vitamin C. Meanwhile, the above extracts all revealed stronger ferric reducing power.

Key words: Balanophora spicata Hayata, antioxidant activity, DPPH free radical scavenging activity, reducing power

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