食品科学 ›› 2010, Vol. 31 ›› Issue (5): 184-189.doi: 10.7506/spkx1002-6300-201005041

• 生物工程 • 上一篇    下一篇

天祝牧区牦牛乳酸奶中优良乳酸菌的分离及筛选

李晓鹏,张卫兵,孙国政,李 静,甘伯中*   

  1. 甘肃农业大学食品科学与工程学院,甘肃农业大学研究测试中心,甘肃省干酪素工程技术研究中心
  • 收稿日期:2009-05-11 修回日期:2009-10-08 出版日期:2010-03-01 发布日期:2010-12-29
  • 通讯作者: 甘伯中 E-mail:ganbz@126.com
  • 基金资助:

    甘肃省高等学校研究生导师科研计划项目(0702-12)

Isolation and Screening of Lactic Acid Bacteria from Yak Yogurt in Tianzhu Pasture Region of Gansu

LI Xiao-peng,ZHANG Wei-bing,SUN Guo-zheng,LI Jing,GAN Bo-zhong*   

  1. College of Food Science and Engineering, Gansu Agricultural University, Analysis and Research Center of Gansu Agricultural
    University, Gansu Casein Engineering and Technology Research Center, Lanzhou 730070, China
  • Received:2009-05-11 Revised:2009-10-08 Online:2010-03-01 Published:2010-12-29
  • Contact: GAN Bo-zhong E-mail:ganbz@126.com

摘要:

从天祝牧区采集的22 份牦牛乳酸奶样品中分离得到96 株乳酸菌。通过初筛:包括遗传稳定性、凝乳时间、滴定酸度、保水率指标的测定及感官评价,得到12 株凝乳时间短、遗传性状稳定的乳酸菌。通过复筛:包括酸化能力、后酸化能力、产乙醛和双乙酰能力、蛋白质分解能力、冷藏期间活菌数、耐受不良环境能力的测定,最终筛选出性能优良的4 株杆菌和2 株球菌。

关键词: 乳酸菌, 分离, 筛选

Abstract:

In this study, 96 strains of lactic acid bacteria (LAB) were isolated from 22 home-made yak yogurt samples collected from Tianzhu pasture region of Gansu. Totally 12 strains of genetically stable lactic acid bacteria were obtained through preliminary selection based on genetic stability, coagulation time, titratable acidity, water-retention rate and sensory evaluation. Finally, 4 lactobacilli and 2 lactococci with excellent performance were selected through the evaluation of acidification ability acetaldehyde-producing ability, diacetyle-producing ability, protein degradation ability, number of living bacteria during cold storage and resistance to adverse environment.

Key words: lactic acid bacteria, isolation, screening

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