食品科学 ›› 2010, Vol. 31 ›› Issue (5): 211-214.doi: 10.7506/spkx1002-6300-201005047

• 生物工程 • 上一篇    下一篇

细菌纤维素高产菌株的筛选及鉴定

曹海鹏,袁 帅,赵 昆,张 霞,胡 承*   

  1. 四川大学生命科学学院,生物资源与生态环境教育部重点实验室
  • 收稿日期:2009-01-19 修回日期:2009-09-02 出版日期:2010-03-01 发布日期:2010-12-29
  • 通讯作者: HU Cheng E-mail:hucheng@scu.edu.cn
  • 基金资助:

    国家微生物资源平台项目(2005DKA21208-7)

Screening and Identification of Cellulose-producing Strains

CAO Hai-peng,YUAN Shuai,ZHAO Kun,ZHANG Xia,HU Cheng*   

  1. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science,
    Sichuan University, Chengdu 610064, China
  • Received:2009-01-19 Revised:2009-09-02 Online:2010-03-01 Published:2010-12-29
  • Contact: HU Cheng E-mail:hucheng@scu.edu.cn

摘要:

以残次的水果为原料,经静置富集培养、多次筛选纯化等步骤,分离出一株细菌纤维素产量较高且遗传性能稳定的菌株G-29。根据第二版《伯杰系统细菌学手册》和第九版《伯杰氏细菌鉴定手册》,对该菌株进行形态、生理生化特征和16S rDNA 分子序列分析。初步鉴定该菌为葡糖醋杆菌属(Gluconacetobacter sp.)一个新菌种或者中间葡糖醋杆菌(Gluconacetobacter intermedius)的一个亚种。菌株G-29 已保藏于中国典型培养物保藏中心,保藏编号为CCTCC NO:M208234。

关键词: 细菌纤维素, 葡糖醋杆菌, 生理生化特征, 鉴定

Abstract:

Wounded fruits were used as raw materials to isolate and obtain a cellulose-producing strain, G-29, with high productivity and genetic stability through static enriching culture and repeated screening. The morphology, physiological and biochemical characteristics and 16S rDNA sequence of G-29 were analyzed. This strain was identified to be a new subspecies of Gluconacetobacter intermedius in the species of Gluconacetobacter. This strain has been preserved in China Center for Typical Culture Collection (CCTCC) with the collection number of CCTCC NO: M208234.

Key words: bacterial cellulose, Gluconacetobacter, physiological and biochemical characteristics, identification

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