关键词: 高效液相色谱法(HPLC), 蒸发光散射检测(ELSD), 食品, 糖

Abstract:

A method for the simultaneous determination of 5 sugars such as fructose, glucose, sucrose, maltose and lactose in foods is described, in which high performance liquid chromatography (HPLC) and evaporative light scattering detection (ELSD) are used. A waters-NH2(4.6 mm × 250 mm, 5 μm) column kept at 30 ℃ and eluted with 75% acetonitrile aqueous solution at a flow rate of 1.0 mL/min was used for the chromatographic separation. The detector drift tube was kept at 45 ℃ and the nitrogen pressure was set at 25 psi. A good separation among the analytes and other components was achieved within 20 min (R ＞ 1.5). The response of the detector to analyte concentration over the range from 50 to 2000μg/mL displayed good linearity. Precision RSDs of 6 replicates for the determination of the sugars were less than 3.8% and mean recoveries for the sugars in 4 real samples spiked at the levels of 100, 200 μg/mL and 500μg/mL were more than 80.3%.

Key words: HPLC, ELSD, food, sugar