食品科学 ›› 2009, Vol. 30 ›› Issue (3): 235-238.doi: 10.7506/spkx1002-6630-200903053

• 生物工程 • 上一篇    下一篇

菲律宾蛤仔多糖提取物抑制蚕豆根尖细胞微核形成的研究

常林瑞,黄清荣,孙振兴,李 清,李爱丽   

  1. 鲁东大学生命科学学院
  • 收稿日期:2008-03-09 修回日期:2008-05-29 出版日期:2009-02-01 发布日期:2010-12-29
  • 通讯作者: 常林瑞 E-mail:linruich189@sohu.com

Study on Inhibitory Effects of Ruditapes philippinarum Polysaccharide against Micronucleus Formation in Vicia faba Root Tip Cells

CHANG Lin-rui,HUANG Qing-rong,SUN Zhen-xing,LI Qing,LI Ai-li   

  1. (College of Life Science, Ludong University, Yantai 264025, China)
  • Received:2008-03-09 Revised:2008-05-29 Online:2009-02-01 Published:2010-12-29
  • Contact: CHANG Lin-rui E-mail:linruich189@sohu.com

摘要:

以菲律宾蛤仔全脏器为原料,经酶解、离心去蛋白、乙醇沉淀、分离、洗涤、干燥得多糖粗制品,通过添加不同浓度的菲律宾蛤仔多糖提取物,研究其对丝裂霉素诱发的蚕豆根尖细胞微核形成的抑制作用。结果表明,浓度为50、100、150μg/ml 的多糖对丝裂霉素诱发的微核均有显著抑制作用,并且随多糖浓度的升高,微核率降低,即抑制效果增强。

关键词: 菲律宾蛤仔多糖, 丝裂霉素, 蚕豆根尖, 微核

Abstract:

Crude polysaccharide was extracted from whole viscera of Ruditapes philippinarum through enzymolysis, centrifugation for removing protein, ethyl alcohol precipitation and drying. Then inhibitory effects of different concentrations of the polysaccharide against micronucleus formation induced by mitomycin C (MMC) in Vicia faba root tip cells were examined. The results showed that 50, 100 and150 μg/ml of polysaccharide can significantly inhibit micronucleus formation induced by MMC. Furthermore, with the increase of polysaccharide concentration, the inhibitory effect is stronger and the rate of micronucleus is lower.

Key words: Ruditapes philippinarum polysaccharide, mitomycin C, Vicia faba root tip, micronucleus

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