食品科学 ›› 2009, Vol. 30 ›› Issue (4): 72-75.doi: 10.7506/spkx1002-6630-200904010

• 工艺技术 • 上一篇    下一篇

春秋桑叶总黄酮含量分析及提取工艺优化

刘 利1,2,潘一乐1,2   

  1. 1.中国农业科学院蚕业研究所 2.江苏科技大学蚕业研究所
  • 收稿日期:2008-01-24 修回日期:2008-09-01 出版日期:2009-02-15 发布日期:2010-12-29
  • 通讯作者: 刘利 E-mail:touchliu@163.com
  • 基金资助:

    国家茧丝绸发展风险基金第五批发展性项目(国茧协办[2003]40 号);
    中国农业科学院蚕业研究所科研发展基金项目(SP200501);江苏科技大学人才项目(2005CY002J)

Analysis and Optimization of Extraction Technology of Total Flavonoids Content in Spring and Autumn Mulberry Leaves

LIU Li1,2,PAN Yi-le1,2   

  1. (1. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China;
    2. Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China)
  • Received:2008-01-24 Revised:2008-09-01 Online:2009-02-15 Published:2010-12-29
  • Contact: LIU Li1,2, E-mail:touchliu@163.com

摘要:

采用分光光度法测定了不同桑种不同种质春、秋桑叶总黄酮含量。分析的12 份种质中,9 份种质秋季桑叶总黄酮含量高于春季,3 份种质春季总黄酮含量高于秋季。鲁桑、广东桑、瑞穗桑、白桑相比较,白桑叶总黄酮含量最低。在单因素试验基础上,通过正交试验,优化了桑叶总黄酮的提取工艺。最佳提取工艺为乙醇浓度70%、提取温度80℃、料液比1:20、提取时间1h。

关键词: 桑叶, 总黄酮含量, 分光光度法, 提取工艺

Abstract:

The total flavonoids content (TFC) in spring and autumn mulberry leaves from different species and germplasm resources were determined by spectrophotometry. Among 12 mulberry germplasm resources, TFC in 9 accessions are higher in autumn than in spring, and other 3 accessions are higher in spring than in autumn. Compared to Morus multicaulis, Morus atropurpurea and Morus mizuho, TFC in Morus alba leaves is the lowest. Moreover, based on single factor tests, the orthogonal tests were used in the optimization of technological parameters. The optimum conditions are as follows: ethanol concentration 70%, 80 ℃, ratio of material to solvent 1:20, and extraction time 1 hour.

Key words: mulberry leaves, total flavonoids content, spectrophotometry, extraction technology

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