沙蒿籽中两种水溶性胶多糖的理化性质与免疫活性研究

doi:10.7506/spkx1002-6630-200907001

食品科学 ›› 2009, Vol. 30 ›› Issue (7): 11-14.doi: 10.7506/spkx1002-6630-200907001

• 基础研究 • 下一篇

沙蒿籽中两种水溶性胶多糖的理化性质与免疫活性研究

郝毓倩¹，孙璇¹，黄琳娟¹，齐春会²，王仲孚^1,*

1.西北大学生命科学学院，西部资源生物与现代生物技术省部共建教育部重点实验室，陕西省生物技术重点实验室 2.军事医学科学院毒物药物研究所

收稿日期:2008-06-25 修回日期:2008-09-03 出版日期:2009-04-01 发布日期:2010-12-29
通讯作者: 王仲孚 E-mail:wangzhf@nwu.edu.cn
基金资助:
“十一五”国家科技支撑计划项目(2006BAD06B00)

Study on separation and purification, physico-chemical properties and immunological activity of two fractions of Water-soluble polysaccharides of Artemisia sphaerocephala Krasch seed

HAO Yu-qian1 SUN Xuan1 HUANG Lin-juan1 QI Chun-hui2 WANG Zhong-fu1,*

(1.Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key
Laboratory of Biotechnology, College of Life Science, Northwest University, Xi an 710069, China 2.Institute of Pharmacology
and Toxicology, The Academy of Military Medical Sciences, Beijing 100850, China)

Received:2008-06-25 Revised:2008-09-03 Online:2009-04-01 Published:2010-12-29
Contact: WANG Zhong-fu1,* E-mail:wangzhf@nwu.edu.cn

摘要/Abstract

摘要：

为研究沙蒿(Artemisia sphaerocephala Krasch)籽中的水溶性多糖成分，经热水提取、醇沉、脱蛋白得到水溶性沙蒿籽胶粗多糖CASPs (crude Artemisia sphaerocephala Krasch seed gum polysaccharides)，CASPs经DEAE-Cellulose柱层析分离得到ASPI、ASPII、ASPIII、ASPIV 四个多糖组分，ASPI经Bio-Gel P-150柱层析纯化后得到ASPI-A，ASPⅢ经Sephadex G-75柱层析得到ASPⅢ-A，ASPⅢ-B，ASPⅢ-C三个组分。气相色谱分析表明，ASPI-A由阿拉伯糖、甘露糖、葡萄糖及半乳糖组成，它们之间的摩尔比为1:2.84:4.89:1.92；ASPⅢ-A由木糖及微量的鼠李糖、阿拉伯糖、葡萄糖、半乳糖、葡萄糖醛酸及半乳糖醛酸组成。高效凝胶渗透色谱(HPGPC)分析表明，ASPI-A为均一性多糖，平均相对分子质量为5.42×104D；ASPⅢ-A的相对分子质量大于4×105D。免疫活性实验结果表明，与Con A合用时，ASPI-A和ASPⅢ-A在一定浓度范围内对小鼠脾细胞增殖反应具有一定的增强活性；ASPⅢ-A在较高浓度时可能具有一定免疫抑制活性。

关键词: 沙蒿籽, 多糖, 分离纯化, 免疫活性

Abstract:

To investigate the components of water-soluble polysaccharides of Artemisia sphaerocephala Krasch seed gum, crude Artemisia sphaerocephala Krasch seed gum polysaccharides (CASP) were isolated through hot water extraction, ethanol precipitation and deproteinazation, and then were fractionated into ASPI, ASPII, ASPIII and ASPIV by DEAE-cellulose column chromatography. ASPI was purified by Bio-Gel P-150 column chromatography and one fraction (ASPI-A) was obtained. ASPIII was fractionated into ASPⅢ-A, ASPⅢ-B and ASPⅢ-C by Sephadex G-75 column chromatography. Monosaccharide analysis by GC showed that the ASPI-A is composed of arabinose, mannose, glucose and galactose with a molar ratio of 1:2.84:4.89:1.92; The ASPIII-A is composed of xylose and slight rhamnose arabinose, glucose, galactose, glucuronic acid and galacturonic acid. High performance gel permeation chromatography (HPGPC) showed that the ASPI-A is homogeneous polysaccharide with an average relative molecular mass of 5.42×104 D and the relative molecular mass of ASPIII-A is more than 4×105 D. The immunological activity assay revealed that in the presence of Con A , ASPI-A and ASPIII-A can increase the splenocyte proliferation in mice in a certain range of concentration; ASPIII-A may have a certain immunosuppression activity in higher concentration.

Key words: Artemisia sphaerocephala Krasch seed gum, polysaccharide, separation and purification, immunological activity

中图分类号:

0629.12

郝毓倩1，孙璇1，黄琳娟1，齐春会2，王仲孚1,*. 沙蒿籽中两种水溶性胶多糖的理化性质与免疫活性研究[J]. 食品科学, 2009, 30(7): 11-14.

HAO Yu-qian1 SUN Xuan1 HUANG Lin-juan1 QI Chun-hui2 WANG Zhong-fu1,*. Study on separation and purification, physico-chemical properties and immunological activity of two fractions of Water-soluble polysaccharides of Artemisia sphaerocephala Krasch seed[J]. FOOD SCIENCE, 2009, 30(7): 11-14.

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沙蒿籽中两种水溶性胶多糖的理化性质与免疫活性研究

Study on separation and purification, physico-chemical properties and immunological activity of two fractions of Water-soluble polysaccharides of Artemisia sphaerocephala Krasch seed

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