关键词: 鸡腿菇多糖, 提取, 响应面分析, Box-Benhnken中心组合实验

Abstract:

Effects of extraction temperature, extraction time, ratio of water to material and extraction times on extraction rate of polysaccharide from Coprinus comatu were investigated respectively in single-factor tests, and then other three key factors except extraction times were optimized through response surface methodology with three factors and three levels based on the principle of Box-Behnken design. The results showed that the optimum extraction temperature, time, ratio of water to material and times are 94.5 ℃, .4 h, 15.5:1 and 2, respectively. Under the above conditions, the extraction rate of polysaccharide from Coprinus comatu reaches 6.16%, which is improved by 7.13% through optimization.

Key words: Coprinus comatus polysaccharide, extraction, response surface methodology, Box-Behnken design