食品科学 ›› 2009, Vol. 30 ›› Issue (15 ): 124-126.doi: 10.7506/spkx1002-6630-200915027

• 基础研究 • 上一篇    下一篇

浒苔提取物抑制食用油脂过氧化能力研究

黄海兰1,徐 可2,王震功2   

  1. 1.青岛大学师范学院 2.青岛第二中学
  • 收稿日期:2009-06-08 出版日期:2009-08-01 发布日期:2010-12-29
  • 通讯作者: 黄海兰1 E-mail:qdhuang07@163.com

Inhibition of Lipid Peroxidation by Extracts from Enteromorpha prolifera (Muell.) J.Ag.

HUANG Hai-lan1,XU Ke2,WANG Zhen-gong2   

  1. HUANG Hai-lan1,XU Ke2,WANG Zhen-gong2
  • Received:2009-06-08 Online:2009-08-01 Published:2010-12-29
  • Contact: HUANG Hai-lan1 E-mail:qdhuang07@163.com

摘要:

用1:1(V/V)的氯仿- 甲醇索氏提取浒苔成分,并用石油醚、乙酸乙酯和正丁醇依次对提取物进行萃取,利用TBARS 法及Schaal 烘箱法进行抗氧化实验,同时与合成抗氧化剂BHT 及没食子酸(GA)进行对照。结果显示:浒苔正丁醇提取物具有较强的抑制亚油酸脂质过氧化的能力,在实验所测4 个浓度(0.4、2、10、50μg/ml)下,均比GA 强,在0.4、2、10μg/ml 浓度时, 强于BHT;同时也具有一定的抑制猪油脂质过氧化的能力,其活性低于BHT。

关键词: 浒苔, 食用油脂, 抗氧化活性

Abstract:

Enteromorpha prolifera (Muell.) J.Ag. (EP) was extracted with a mixture chloroform and methanol (1:1) in a Soxhlet extractor, and then dispersed in water and partitioned with petroleum ether, ethyl acetate and n-butanol successively. The antioxidant activity (AA) of both chloroform-methanol extract and its four fractions including petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (BF) and aqueous residue (AR) was evaluated by TBARS and Schaal methods, and compared with that of butylated hydroxytoluene (BHT) and gallic acid (GA). Fraction BF exhibited higher inhibition activity on linoleic acid peroxidation than GA at 0.4, 2, 10 and 50 μg /ml and than BHT at 0.4, 2 and 10 μg /ml while lower inhibition activity on lard peroxidation than BHT at the same concentration levels tested.

Key words: Enteromorpha prolifra (Muell.) J.Ag., edible oil and fat, lipid peroxidation

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