食品科学 ›› 2009, Vol. 30 ›› Issue (16 ): 106-110.doi: 10.7506/spkx1002-6630-200916018

• 工艺技术 • 上一篇    下一篇

罗非鱼皮胶原蛋白的提取条件优化及性质

杨贤庆1,张 帅1,2,郝淑贤1,李来好1,吴燕燕1,石 红1,黄 卉1   

  1. 1.中国水产科学研究院南海水产研究所 2.上海海洋大学
  • 收稿日期:2009-04-26 出版日期:2009-08-15 发布日期:2010-12-29
  • 通讯作者: 杨贤庆 E-mail:yxqgd@163.com
  • 基金资助:

    农业部2007 年公益性行业(农业)科研专项(3-49);广东省农业重点项目(2008A020100006;2009B020201003);广东省海洋渔业科技推广专项(A200899B02)

Optimization of Extraction and Physico-chemical Properties of Tilapia Skin Collagen

YANG Xian-qing1,ZHANG Shuai1,2,HAO Shu-xian1,LI Lai-hao1,WU Yan-yan1,SHI Hong1,HUANG Hui1   

  1. 1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
    2. Shanghai Ocean University, Shanghai 200090, China
  • Received:2009-04-26 Online:2009-08-15 Published:2010-12-29
  • Contact: YANG Xian-qing E-mail:yxqgd@163.com

摘要:

采用Box-Benhnken 中心组合试验设计及响应面分析法优化热水提取罗非鱼鱼皮胶原蛋白工艺,得到最佳提取工艺为:4℃条件下用0.213mol/L 盐酸浸泡鱼皮21min(料液比1:20,m/V),蒸馏水浸泡(料液比1:7,m/V),42℃水浴提取12.6h,最终得到的胶原蛋白含量达293.018mg/g,产品甘氨酸、脯氨酸和羟脯氨酸含量丰富。SDSPAGE实验结果表明胶原蛋白中含有α1、α2 和β链,是典型的Ⅰ型胶原蛋白,凝胶强度为682g,特性黏度为1.461dl/g。

关键词: 罗非鱼鱼皮, 胶原蛋白, 热水提取, 凝胶强度, 黏度

Abstract:

The hot water extraction of collagen from tilapia skin was optimized using Box-Behnken design and response surface methodology and the collagen was analyzed for its physicochemical properties. The optimum conditions for the improved extraction of collagen from tilapia skin were determined as follows: soaking tilapia skin in 0.213 mol/L HCl solution with a ratio of material to liquid of 1:20 (m/V) at 4 ℃ for 21 min prior to the hot water extraction with a ratio of material to liquid of 1:7 (m/V) at 42 ℃ for 12.6 h, and the optimized extraction led to a collagen content of 293.018 mg/g. The collagen was rich in glycine, proline and hydroxyproline. SDS-PAGE analysis showed that this collagen contained α1, α2 and β chains indicating that it is a typical type I collagen. Its gel strength and characteristic viscosity were 682 g and 1.461dl/g, respectively.

Key words: tilapia skin, collagen, hot water extraction, gel strength, characteristic viscosity

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