食品科学 ›› 2009, Vol. 30 ›› Issue (16 ): 131-134.doi: 10.7506/spkx1002-6630-200916024

• 工艺技术 • 上一篇    下一篇

新疆野生苍耳叶中总黄酮的超声波提取工艺研究

阿布力克木·阿布力孜,阿布力米提·阿布都卡德尔,迪丽努尔·塔力甫*   

  1. 新疆大学化学与化工学院
  • 收稿日期:2009-04-27 出版日期:2009-08-15 发布日期:2010-12-29
  • 通讯作者: 迪丽努尔·塔力甫 E-mail:dilnurt@yahoo.com.cn
  • 基金资助:

    新疆大学校院联合项目(XY080112)

Ultrasonic-assisted Extraction of Total Flavonoids from Leaves of Wild Xanthium L. Grown in Xinjiang

ABULIKEMU Abulizi,ABULIMITI Abudoukadeer,DILINUER Talipu*   

  1. College of Chemistry and Chemical Engineering, Xinjiang University, Urumqi 830046, China
  • Received:2009-04-27 Online:2009-08-15 Published:2010-12-29
  • Contact: DILINUER Talipu E-mail:dilnurt@yahoo.com.cn

摘要:

以新疆野生苍耳叶为原料,利用超声波提取黄酮类化合物。在单因素试验的基础上,采用正交试验法,考察乙醇体积分数、料液比、超声温度、超声时间对总黄酮得率的影响,并用紫外可见分光光度法测定苍耳叶中总黄酮得率,最终确定最佳提取工艺条件为乙醇体积分数50%、料液比1:50、超声温度30℃、超声时间30min。在该最佳条件下,总黄酮得率为3.10mg/g,该方法采用全物理过程,无任何污染,是提取苍耳叶中黄酮类物质的有效途径。

关键词: 苍耳叶, 总黄酮, 超声波法提取, 提取工艺

Abstract:

An ultrasonic-assisted extraction of total flavonoids from leaves of wild Xanthium L. grown in Xinjiang was developed. Single-factor method was adopted to investigate the effects of ethanol concentration, extraction temperature, ultrasonic treament time and material/liquid ratio on the extraction yield of total flavonoids. Subsequently, a four-variable/three-level orthogonal array design was carried out to maximize the extraction of total flavonoids. An extraction yield of total flavonoids of 3.10 mg/g was attained under the optimum extraction conditions as follows: ethanol concentration 50%, extraction temperature 30 ℃, ultrasonic treatment time 30 min, and material/liquid ratio 1:50. The ultrasonic-assisted extraction can be regarded as a purely physical process without any pollution, thus providing an ideal approach to extracting flavonoids from leaves of Xanthium L.

Key words: leaves of Xanthium L., total flavonoids, ultrasonic-assisted extraction, extraction process

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