免疫初乳对腹泻大鼠血浆脂肪酸和葡萄糖及肠黏膜sIgA 的影响

doi:10.7506/spkx1002-6630-200919071

食品科学 ›› 2009, Vol. 30 ›› Issue (19 ): 300-303.doi: 10.7506/spkx1002-6630-200919071

免疫初乳对腹泻大鼠血浆脂肪酸和葡萄糖及肠黏膜sIgA 的影响

高伟¹，许龙兵¹，潘道东²，陈龙^1,3,*

1.南京师范大学生命科学学院 2.南京师范大学金陵女子学院食品科学系 3.江苏省生物医药功能材料工程研究中心

收稿日期:2009-05-07 出版日期:2009-10-01 发布日期:2010-12-29
通讯作者: 陈龙1,3,* E-mail:lchen@njnu.edu.cn
基金资助:
“十五”国家奶业重大科技专项项目(2002BA518A12)；江苏省“十五”攻关项目(BE2002302)

Effects of Immune Colostrum on Plasma Fatty Acid and Glucose and Intestinal Mucosa sIgA in Diarrhetic Rats

GAO Wei1，XU Long-bing1，PAN Dao-dong2，CHEN Long1,3,*

1. College of Life Sciences, Nanjing Normal University, Nanjing 210046, China；
2. Department of Food Sciences, Ginling College, Nanjing Normal University, Nanjing 210097, China；
3. Jiangsu Engineering Research Center for Bio-medical Function Materials, Nanjing 210097, China

Received:2009-05-07 Online:2009-10-01 Published:2010-12-29
Contact: CHEN Long1,3,* E-mail:lchen@njnu.edu.cn

摘要/Abstract

摘要：

目的：探讨免疫初乳在干预急性感染性腹泻病中对营养代谢的调节作用及其机制。方法：选用32 只健康成年SD 大鼠，随机分成对照组(C 组)、模型组(M 组)、普通初乳保护组(NC 组)、免疫初乳保护组(IC 组)。NC和IC 组每天分别灌服普通初乳和免疫初乳(1ml/100g bw)，C 和M组灌服生理盐水，连续12d。在灌服实验后的5、10d，M、NC 和IC 组大鼠感染混合的肠侵染性大肠杆菌和沙门氏菌菌液(1 × 108ml/100g bw)。结果：在实验的第12 天，M 组和NC 组大鼠血浆游离脂肪酸(free fatty acid，FFA)水平分别显著低于和高于对照组，同时M 组和NC 组大鼠血浆葡萄糖(glucose，Glu)水平分别显著高于对照组，而IC 组和对照组无显著差异。此时，NC 组大鼠血清血浆胰岛素(insulin，Ins)水显著高于M、IC 组。M组和NC 组大鼠小肠液免疫球蛋白A(sIgA)水平分别显著高于和低于对照组，而IC 组和对照组无显著差异。结论：免疫初乳通过抗大鼠感染性腹泻的保护效应，能够减少肠道病原菌对肠黏膜免疫系统的刺激，调整和维持机体内Glu 和FFA 含量的相对稳定。

关键词: 免疫初乳, 大鼠, 腹泻, 胰岛素, 游离脂肪酸, 葡萄糖, s I g A

Abstract:

Objectives: To explore regulation effects and mechanisms of immune colostrum on nutrition and metabolism in acute infectious diarrhea diseases. Methods: Thirty-two healthy adult SD rats with half of female and male were randomly divided into a control group (GC), a model group (GM), a group protected with normal colostrum (GNC) and a group protected with immune colostrum (GIC). Rats in GNC and GIC were orally administered with normal and immune colostrum at a dose of 1 ml/100 g body weight once a day for 12 days, respectively. Rats in GC and GM were administered saline using the same methods as controls. At 5 and 10 d post-experiment, the rats in GM, GNC and GIC, respectively, were orally infected with mixed enteroinvasive strains containing 1×108/ml of E. coli and Salmonella typhi at a dose of 1 ml/100g body weight once. Results: Free fatty acid (FFA) level in plasma of GM was obviously lower than that of GC; in contrast, FFA level in plasma of GNC was significantly higher than that of GC. Meanwhile, glucose in plasma of both GM and GNC was obviously higher than that of GC. However, no significant difference was observed between GIC and GC. At the same time, a significantly elevated insulin level in serum of GNC was detected compared with GM and GNC. The sIgA level exhibited an increase in GM and a reduction in GNC compared with GC, but no obvious difference was revealed between GIC and GC. Conclusion: Immune colostrum may attenuate stimulations from diarrhea pathogen bacteria on intestinal mucosa immune system to regulate and maintain a relative stabilization of Glu or FFA content and effectively protect anti-infectious diarrhea in rats.

Key words: immune colostrums, rat, diarrhea, insulin, free fatty acid, glucose, sIgA

中图分类号:

R392

高伟1，许龙兵1，潘道东2，陈龙1,3,*. 免疫初乳对腹泻大鼠血浆脂肪酸和葡萄糖及肠黏膜sIgA 的影响[J]. 食品科学, 2009, 30(19 ): 300-303.

GAO Wei1，XU Long-bing1，PAN Dao-dong2，CHEN Long1,3,*. Effects of Immune Colostrum on Plasma Fatty Acid and Glucose and Intestinal Mucosa sIgA in Diarrhetic Rats[J]. FOOD SCIENCE, 2009, 30(19 ): 300-303.

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免疫初乳对腹泻大鼠血浆脂肪酸和葡萄糖及肠黏膜sIgA 的影响

Effects of Immune Colostrum on Plasma Fatty Acid and Glucose and Intestinal Mucosa sIgA in Diarrhetic Rats

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