食品科学 ›› 2010, Vol. 31 ›› Issue (6): 52-54.doi: 10.7506/spkx1002-6630-201006012

• 工艺技术 • 上一篇    下一篇

刺叶提灯藓花生四烯酸提取与含量测定

王桂花,谢树莲*   

  1. 山西大学生命科学与技术学院
  • 收稿日期:2009-05-25 修回日期:2009-10-23 出版日期:2010-03-15 发布日期:2010-12-29
  • 通讯作者: 谢树莲 E-mail:xiesl@sxu.edu.cn
  • 基金资助:

    山西省科技厅农业攻关项目(2007031042)

Extraction and Determination of Arachidonic Acid in Mnium spinosum

WANG Gui-hua,XIE Shu-lian*   

  1. School of Life Science and Technology, Shanxi University, Taiyuan 030006, China
  • Received:2009-05-25 Revised:2009-10-23 Online:2010-03-15 Published:2010-12-29
  • Contact: XIE Shu-lian* E-mail:xiesl@sxu.edu.cn

摘要:

目的:提取刺叶提灯藓(Mnium spinosum)中的花生四烯酸并测定其含量。方法:采用柱层析法从刺叶提灯藓植株中分离提取,并用高效液相色谱法测定其花生四烯酸含量。结果:刺叶提灯藓的乙醇萃取液中花生四烯酸含量较高达6.5%,而石油醚和正己烷萃取液中未测出。结论:刺叶提灯藓中花生四烯酸含量可观,以乙醇萃取液提取效果最佳。

关键词: 花生四烯酸, 刺叶提灯藓, 高效液相色谱法, 柱层析法

Abstract:

Mnium spinosum (Voit) Schwagr was extracted with different organic solvents in Soxhlet extractor. The resulting extracts were then purified by silica column chromatography for analyzing arachidonic acid (AA) content by HPLC. Results showed that ethanol extract of Mnium spinosum (Voit) Schwagr exhibited an AA content of 6.5%, while no AA wad detected in petroleum ether extract and n-hexane extract of Mnium spinosum (Voit) Schwagr. Thus, ethanol is superior to other two solvents for extracting considerable AA from Mnium spinosum (Voit) Schwagr.

Key words: arachidonic acid, Mnium spinosum (Voit) Schwagr, high performance liquid chromatography (HPLC), column chromatography

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