食品科学 ›› 2010, Vol. 31 ›› Issue (9): 229-233.doi: 10.7506/spkx1002-6630-201009051

• 营养卫生 • 上一篇    下一篇

琐琐葡萄多糖对小鼠免疫功能的影响

俞 丹1,赵 军2,刘 涛1,苏德奇1,马 龙1,*   

  1. 1. 新疆医科大学公共卫生学院卫生毒理教研室 2. 新疆药物研究所维吾尔药研究开发重点实验室
  • 收稿日期:2009-07-01 出版日期:2010-05-01 发布日期:2010-12-29
  • 通讯作者: 马 龙 E-mail:ydwwl@163.com
  • 基金资助:

    国家自然科学基金项目(30660157)

Effect of Polysaccharides from Vitis viniferal L. on Immune Function in Mice

YU Dan1,ZHAO Jun2,LIU Tao1,SU De-qi1,MA Long1,*   

  1. 1. Department of Toxicology, College of Public Health, Xinjiang Medical University, Urumqi 830011, China;2. Xinjiang Key
    Laboratory for Research and Development of Uighur Drugs, Institute of Materia Medica of Xinjiang, Urumqi 830002, China
  • Received:2009-07-01 Online:2010-05-01 Published:2010-12-29
  • Contact: MA Long E-mail:ydwwl@163.com

摘要:

目的:探讨琐琐葡萄多糖(VTP)对小鼠免疫功能的影响。方法:经热水提取、乙醇沉淀提取得到VTP,采用苯酚- 硫酸法测定多糖含量,应用环磷酰胺制备免疫低下小鼠模型,通过测定给药后小鼠脾脏及胸腺指数、抗体分泌细胞功能、血清溶血素水平和脾淋巴细胞转化功能,研究VTP 对免疫低下小鼠免疫功能的影响。结果:提取得到VTP 粗品并测得其中多糖含量为20.68%,RSD 为1.93%;与模型组相比,VTP 可增加免疫低下模型小鼠脾脏的质量,有效促进小鼠脾淋巴细胞的转化功能,明显改善小鼠抗体分泌细胞的功能,并可使红细胞半数溶血值显著增高。结论:VTP 对免疫低下模型小鼠的体液免疫功能和细胞免疫功能具有一定的保护作用。

关键词: 琐琐葡萄, 多糖, 免疫功能

Abstract:

Objectives: To study the effect of polysaccharides from Vitis viniferal L. (VTP) on immune function in mice. Methods: VTP was extracted using water and precipitated using 95% ethanol. The content of VTP was assayed with phenol-vitriolic colorimetry. Immunosuppressed mice were induced by cyclophosphamide and their indices of thymus and spleen, antibodysecreting cell function, serum hemolysin level and lymphocyte transformation capability were measured. Results: The content of polysaccharides in VTP crude extract was 20.68% with the RSD of 1.93%. Compared with the model group, VTP could increase spleen weight of mice, significantly promote lymphocyte transformation capability and antibody-secreting cell function, and improve serum hemolysin level. Conclusion: VTP could improve both humoral immune function and cellular immune function of immunosuppressed mice.

Key words: Vitis viniferal L., polysaccharides, immune function

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