关键词: 猪胰脂肪酶, 花椒籽油, 水解, 动力学, 条件优化

Abstract:

In order to exploit α-linolenic acid from prickly ash seed oil, the hydrolysis characteristics of prickly ash seed oil by porcine pancreatic lipase were investigated and some key hydrolysis conditions were explored. The results indicated that an equation expressing oil-water interface (αt) with respect to reaction temperature (t), agitation speed (ω) and substrate concentration (S) was developed as follows: αt= 0.04ω 0.577t1.242S (1 + 0.02S). The change in hydrolysis reaction rate with varying reaction temperature obeyed Arrhenius equation. The activation energy was 21.891 kJ/mol. Based on Michaelis-Menten equation and the effect of αt on reaction rate, a kinetic model was established to describe the hydrolysis of prickly ash seed oil by porcine pancreatic lipase. The optimal hydrolysis conditions of prickly ash seed oil by porcine pancreatic lipase were reaction temperature of 50 ℃, oil concentration of 30%, enzyme concentration of 72.9 g/L and reaction time of 4 h. The dgree of hydrolysis reached up to 57.13% under these optimal conditions.

Key words: porcine pancreatic lipase, prickly ash seed oil, hydrolysis, kinetic, condition optimization