食品科学 ›› 2010, Vol. 31 ›› Issue (20): 260-265.doi: 10.7506/spkx1002-6630-201020052

• 工艺技术 • 上一篇    下一篇

酶法水解香/芭蕉根部球茎粉浆研究及动力学分析

陈 伟,黄广民*   

  1. 海南大学食品学院
  • 收稿日期:2010-06-22 修回日期:2010-09-26 出版日期:2010-10-25 发布日期:2010-12-29
  • 通讯作者: 黄广民 E-mail:hgmin886699@163.com
  • 基金资助:

    海南省教育厅高校科研项目(Hj200710);海口市重点科技项目(0000017)

Optimal Conditions and Kinetics of Enzymatic Hydrolysis of Banana Corm Powder

CHEN Wei,HUANG Guang-min*   

  1. College of Food Science and Technology, Hainan University, Haikou 570228, China
  • Received:2010-06-22 Revised:2010-09-26 Online:2010-10-25 Published:2010-12-29
  • Contact: HUANG Guang-min E-mail:hgmin886699@163.com

摘要:

考察香/ 芭蕉根部球茎粉浆酶法水解液化过程,采用液态高温α- 淀粉酶,在高温条件下作用于香/ 芭蕉根部球茎粉浆,通过单因素和正交试验,分别检测水解液中还原糖含量,以考察其作用情况。结果表明:液化温度85℃、粉浆pH6.4、香/ 芭蕉根部球茎粉浆质量浓度30mg/mL、加酶量0.064mL/g,酶法水解液化效果最佳,Vm 为0.708mg/(mL·min),Km 为27.410mg/mL,水解液的DE 值达到37.61%。

关键词: 香/ 芭蕉根部球茎, 高温α- 淀粉酶, 动力学

Abstract:

The optimal conditions for the hydrolysis of banana corm powder with thermostable alpha-amylase were investigated by single-factor and orthogonal array design methods, and a kinetic analysis of the hydrolysis of banana corm powder was performed. Liquefaction temperature of 85 ℃, pH of 6.4, substrate concentration of 30 mg/mL and enzyme loading of 0.064 mL/g were found optimal, and a DE value of 37.61% was obtained under these conditions. The Vm and Km were determined to be 0.708mg/ (mL·min) and 27.410 mg / mL, respectively.

Key words: banana root corm, thermostable alpha-amylase, kinetics

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