食品科学 ›› 2010, Vol. 31 ›› Issue (21): 279-282.doi: 10.7506/spkx1002-6630-201021063

• 生物工程 • 上一篇    下一篇

可降解L- 苹果酸和柠檬酸菌株的筛选及鉴定

王立芳,张 微,文连奎*   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2010-07-05 修回日期:2010-10-21 出版日期:2010-11-15 发布日期:2010-12-29
  • 通讯作者: 文连奎 E-mail:wenliankui@163.com
  • 基金资助:

    吉林省科技厅重点项目(20090228)

Screening and Identification of a Strain Degrading L-Malic Acid and Critic Acid

WANG Li-fang,ZHANG Wei,WEN Lian-kui*   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Received:2010-07-05 Revised:2010-10-21 Online:2010-11-15 Published:2010-12-29
  • Contact: WEN Lian-kui E-mail:wenliankui@163.com

摘要:

从葡萄园土壤中分离得到一株可降解L- 苹果酸和柠檬酸的菌株,通过对其进行降酸能力实验,测得该菌株对12g/L 的L- 苹果酸和柠檬酸的降解率分别达到93.17% 和92.08%。对菌株进行形态学、生理生化鉴定及rDNA-ITS(internal transcribed spacer,内转录间隔区)序列分析,鉴定该菌株为伊萨酵母属(Issatchenkia)陆生伊萨酵母种(I.terricola)。对其生物学特性进行研究,发现其最适生长温度为30℃,最适生长pH 值为2.0~5.5。经传代培养10 代,其降酸性能和生长特性仍较稳定。

关键词: 降解, L-苹果酸, 柠檬酸, 筛选, 鉴定

Abstract:

A strain (named S8) degrading both L-malic acid and critic acid was isolated from the soil of the wineyard in Jilin Agricultural University. The abilities of this strain to degrading 12 g/L L-malic acid and critic acid solutions were measured, and deacidification rate was 93.17% for L-malic acid and 92.08% for critic acid. Based on the morphological, physiological and biochemical identification and the rDNA-ITS (internal transcribed spacer) sequence analysis, this stain was classified as Issatchenkia terricola. The investigation of its biological characteristics showed that the optimal temperature and pH for its growth were 30 ℃ and 2.0-5.5, respectively. After passaging of 10 generations, the acid-degrading ability and growth characteristics of this strain were still stable.

Key words: degradation, L-malic acid, critic acid, screening, identification

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