食品科学 ›› 2011, Vol. 32 ›› Issue (1 ): 158-161.doi: 10.7506/spkx1002-6630-201101038

• 生物工程 • 上一篇    下一篇

酿酒酵母生产谷胱甘肽分批发酵动力学研究

郑丽雪,王立梅,梅艳珍,齐 斌*   

  1. 1. 常熟理工学院,生物与食品工程学院
  • 收稿日期:2010-03-25 修回日期:2010-12-15 出版日期:2011-01-15 发布日期:2010-12-28
  • 通讯作者: 齐 斌* E-mail:qibin65@126.com
  • 基金资助:

    2008 年江苏省高校“青蓝工程”中青年学术带头人资助项目;苏州市科技发展计划应用基础研究项目(YJG0913);常熟理工学院青年教师科研启动项目(QZ0914)

Batch Fermentation Kinetics for Glutathione Production by Saccharomyces cerevisiae

ZHENG Li-xue,WANG Li-mei,MEI Yan-zhen,QI Bin*   

  1. College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Received:2010-03-25 Revised:2010-12-15 Online:2011-01-15 Published:2010-12-28
  • Contact: QI Bin* E-mail:qibin65@126.com

摘要:

以麦芽汁为发酵培养基,对7.5L 自动发酵罐中酿酒酵母Y518 分批发酵生产谷胱甘肽的实验数据进行分析,建立谷胱甘肽分批发酵动力学模型。通过对符合菌体生长的Logistic 方程、产物生成的Luedeking-Piret 方程和基质消耗的物料衡算方程进行最优参数估计和非线性拟合,分别得到了相应的动力学模型和最佳模型参数值。对动力学模型的拟合曲线进行分析,发现模型的计算值与实验值能较好地拟合,表明本实验建立的分批发酵动力学模型能较好地反映谷胱甘肽分批发酵过程。

关键词: 谷胱甘肽, 麦芽汁, 酿酒酵母, 发酵动力学模型, 拟合

Abstract:

A kinetic model of glutathione production by batch fermentation with Saccharomyces cerevisiae Y518 was studied by using malt sprout extract as the carbon source in a 7.5 L fermenter. The kinetic models for cell growth, glutathione production and substrate consumption were developed respectively by Logistic equation, Luedeking-Piret equation and materials balance equation through optimal parameter estimation and nonlinear fitting. The optimal set of parameters was estimated by fitting the model to experimental data. The results predicted by the model were in good agreement with the experimental observations and showed that the models could provide a reasonable description for the batch fermentation of glutathione.

Key words: glutathione, malt sprout extract, Saccharomyces cerevisiae, fermentation kinetic model, fitting

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