食品科学 ›› 2011, Vol. 32 ›› Issue (6 ): 1-6.doi: 10.7506/spkx1002-6630-201106001

• 工艺技术 •    下一篇

普洱茶茶色素提取工艺条件的响应面分析及其抗氧化性活性研究

杨新河1,2,李 勤1,3,黄建安1,3,陆 英1,3,沈 智1,3,刘仲华1,3,*   

  1. 1.国家植物功能成分利用工程技术研究中心 2.孝感学院生命科学技术学院 3.湖南农业大学 茶学教育部重点实验室
  • 收稿日期:2010-04-22 修回日期:2011-01-18 出版日期:2011-03-25 发布日期:2011-03-03
  • 通讯作者: 杨新河 E-mail:yangxinhe@163.com
  • 基金资助:
    “十一五”国家科技支撑计划重大项目(2007BAD58B00-03);云南省政府茶叶专项(2007YNCXB-01-01)

Optimization of Process Conditions for Pu-erh Tea Pigment Extraction by Response Surface Analysis and Its Antioxidant Activity

YANG Xin-he1,2,LI Qin1,3,HUANG Jian-an1,3,LU Ying1,3,SHEN Zhi1,LIU Zhong-hua1,3,*   

  1. 1. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingedients, Changsha 410128,
    China ;2. College of Life Science and Technology, Xiaogan University, Xiaogan 432000, China ;3. Key Laboratory of Tea
    Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China
  • Received:2010-04-22 Revised:2011-01-18 Online:2011-03-25 Published:2011-03-03
  • Contact: YANG Xin-he1 E-mail:yangxinhe@163.com

摘要: 目的:优化普洱茶茶色素的提取工艺及研究其抗氧化性活性。方法:以与蒸馏水的色差作为普洱茶茶色素提取的评价指标,在单因素试验基础上,利用Box-Behnken中心组合试验和响应面分析法,对普洱茶茶色素提取工艺参数进行优化;以清除1,1-二苯基-2-苦基肼自由基(DPPH自由基)的能力评价普洱茶茶色素的抗氧化性活性。结果:普洱茶茶色素提取的最佳工艺条件为液料比45:1(mL/g)、提取温度100℃、提取时间150min、色差52.97,高出单因素试验和Box-Behnken组合中的最高色差4.73%;普洱茶茶色素清除DPPH自由基的IC50值30.83μg/mL。结论:采用响应曲面法对普洱茶茶色素提取条件进行优化可行;普洱茶茶色素具有良好的抗氧化作用,可作为天然抗氧化剂进一步开发和利用。

关键词: 普洱茶, 茶色素, 提取, 响应面分析, 抗氧化性活性

Abstract: Objective: To optimize the extraction process for Pu-erh tea pigment and explore its antioxidant activity. Methods: Following single factor investigations, optimization of process conditions for the extraction of Pu-erh tea pigment based on the color difference value E between distilled water and Pu-erh tea pigment was carried out using central design composite design combined with response surface methodology (RSM). 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the antioxidant activity of the pigment. Results: The optimal extraction conditions for Pu-erh tea pigment were liquid-to-material ratio of 45:1 (mL/g), extraction temperature of 100 ℃ and extraction time of 150 min. Under the optimal extraction conditions, a color difference E value of 52.97 was obtained, which revealed 4.73% increase as compared to the highest color difference in single-factor investigations and Box-Behnken composite experiments. The IC50 value for the scavenging capability of Pu-erh tea pigment on DPPH free radicals was 30.83μg/mL. Conclusion: It is feasible to optimize the extraction of Pu-erh tea pigment by response surface methodology. The Pu-erh tea pigment with excellent antioxidant activity has the potential to be further developed and utilized as a natural antioxidant.

Key words: Pu-erh tea, tea pigment, extraction, response surface methodology, antioxidant activity

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