食品科学 ›› 2011, Vol. 32 ›› Issue (7 ): 183-187.doi: 10.7506/spkx1002-6630-201107039

• 生物工程 • 上一篇    下一篇

地衣芽孢杆菌产Levan果聚糖发酵条件的优化

陆娟1.2,肖敏1,卢丽丽1   

  1. 1.山东大学微生物技术国家重点实验室,国家糖工程技术研究中心 2.阜阳师范学院生命科学学院
  • 收稿日期:2010-07-11 修回日期:2011-02-22 出版日期:2011-04-15 发布日期:2011-03-30
  • 通讯作者: 肖敏 E-mail:minxiao@sdu.edu.cn
  • 基金资助:
    国家自然科学基金项目(31070064);山东大学自主创新基金项目(2009DX002)

Optimization of Fermentation Conditions for Production of Levan by Bacillus licheniformis 8-37-0-1

 LU Juan,XIAO Min,LU Li-li   

  1. 1.State Key Laboratory of Microbial Technology and National Glycoengineering Research Center,Shandong University, Jinan 250100,China;2.School of Life Sciences,Fuyang Teachers College,Fuyang 236041,China
  • Received:2010-07-11 Revised:2011-02-22 Online:2011-04-15 Published:2011-03-30

摘要: 通过单因素试验(培养基用水、碳源、氮源、培养温度和培养基初始pH值)和正交试验对地衣芽孢杆菌(Bacillus licheniformis)8-37-0-1发酵产生Levan果聚糖的培养基组成及培养条件进行优化,采用苯酚-硫酸法测定多糖含量。结果表明:以蔗糖100g/L、牛肉膏1.0g/L、酵母粉0.6g/L、K2HPO4 3.0g/L、KH2PO4 3.0g/L、NaCl 1.0g/L、MgSO4·7H2O 0.2g/L、FeSO4·7H2O 0.001g/L,自来水配制,培养基初始pH5.0,30℃培养8-37-0-1菌株24h,Levan果聚糖产量达到最高值41.7g/L,约是未优化时的5.0倍。

关键词: Bacillus licheniformis 8-37-0-1, Levan, 发酵条件, 优化

Abstract: The medium and fermentation conditions for levan production by Bacillus licheniformis 8-37-0-1 were optimized by single factor and orthogonal array experiments. After culture at 30 ℃ for 24 h in a medium prepared with tap water consisting of sucrose 100 g/L, yeast extract 0.6 g/L, beef extract 1.0 g/L, KH2PO4 3.0 g/L, K2HPO4 3.0 g/L, NaCl 1.0 g/L, MgSO4·7H2O 0.2 g/L and FeSO4·7H2O 0.001 g/L, a maximum levan production of 41.7 g/L was achieved, which was 5.0 times higher than before optimization.

Key words: Bacillus licheniformis 8-37-0-1, levan, fermentation condition, optimization

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