食品科学 ›› 2011, Vol. 32 ›› Issue (8): 121-124.doi: 10.7506/spkx1002-6630-201108027

• 工艺技术 • 上一篇    下一篇

微波辅助蛋白质水解效果稳定性研究

杜雪莉1,张凤枰1,2,3,刘耀敏1,张凌晶4,*   

  1. 1. 通威股份有限公司
    2.中国科学院西北高原生物研究所 3.中国科学院研究生院 4.集美大学生物工程学院
  • 出版日期:2011-04-25 发布日期:2011-04-12
  • 基金资助:
    福建省高校水产科学技术与食品安全重点实验室基金项目(20095402)

Stability of Microwave-assisted Acid Hydrolysis of Protein

DU Xue-li1,ZHANG Feng-ping1,2,3,LIU Yao-min1,ZHANG Ling-jing4,*   

  1. 1. Tongwei Co. Ltd., Chengdu 610041, China;2. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China;3. Graduate University of the Chinese Academy of Sciences,Beijing 100049, China;4. College of Biotechnology, Jimei University, Xiamen 361021, China
  • Online:2011-04-25 Published:2011-04-12

摘要: 目的:考察微波辅助蛋白质水解效果的稳定性。方法:以鱼粉和豆粕为研究对象,采用微波辅助蛋白质水解-柱后衍生-紫外检测法测定氨基酸,通过比较分析一定时期内鱼粉和豆粕样品氨基酸结果的变化,研究微波辅助蛋白质水解效果的稳定性。结果:在一定时期内,采用微波辅助蛋白质水解,进口鱼粉、豆粕样品氨基酸测定结果RSD值均小于4.0%。结论:微波辅助蛋白质水解效果的稳定性好,可替代传统蛋白质酸水解方法,并为保证氨基酸检测结果的准确性提供技术支持。

关键词: 氨基酸分析, 微波辅助蛋白水解, 稳定性

Abstract: In this study, fish meal and soybean meal were used as substrate to compare microwave-assisted acid hydrolysis and routine acid hydrolysis by determining the amino acid composition of hydrolysates on an auto-amino acid analyzer. Meanwhile, in order to evaluate the stability of microwave-assisted acid hydrolysis, two imported fish meal samples and one soybean meal sample were subjected to microwave-assisted acid hydrolysis and amino acid determination (the same samples were hydrolyzed and analyzed at four different dates). The results showed that the relative standard errors for the determination of each of the three samples at different dates were less than 4%. Therefore, microwave-assisted acid hydrolysis has a good stability and can be used as an alternative to routine acid hydrolysis and provide technical supports for accurate determination of amino acids.

Key words: amino acid analysis, microwave-assisted acid hydrolysis of protein, stability

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