野生山核桃油对去卵巢大鼠抗氧化能力与海马细胞凋亡的影响

doi:10.7506/spkx1002-6630-201109060

食品科学 ›› 2011, Vol. 32 ›› Issue (9): 272-275.doi: 10.7506/spkx1002-6630-201109060

野生山核桃油对去卵巢大鼠抗氧化能力与海马细胞凋亡的影响

陈默然，沈楠，雷钧涛，赵行宇

1.吉林医药学院科研处 2.吉林医药学院实验中心 3.吉林医药学院药学教研室 4.吉林医药学院心理教研室

出版日期:2011-05-15 发布日期:2011-04-11
基金资助:
吉林省教育厅“十一五”科学技术项目(2008-265)

Effects of Wild Walnut Oil on Antioxidant Functions in Ovariectomized Rats and Apoptosis of Hippocampal Cells

CHEN Mo-ran1，SHEN Nan2，LEI Jun-tao3， ZHAO Xing-yu4,*

1. Research Division, Jilin Medical College, Jilin 132013, China；2. Center of Experiment, Jilin Medical College, Jilin 132013, China；3. Department of Pharmacy, Jilin Medical College, Jilin 132013, China；4. Department of Psychology, Jilin Medical College, Jilin 132013, China

Online:2011-05-15 Published:2011-04-11

摘要/Abstract

摘要： 目的：观察野生核桃油对机体抗氧化能力及海马细胞凋亡的影响。方法：以去卵巢大鼠为老年动物模型，每日灌胃给予野生山核桃油(2.4、1.2、0.6g/kg)，用放免法(RIA)检测血清雌二醇(E2)的含量；用生化法测定海马匀浆液总超氧化物歧化酶(T-SOD)、丙二醛(MDA)的水平；免疫组化观察海马齿状回Bcl-2与Bax的表达；Hoechst染色观察海马齿状回细胞的凋亡水平。结果：与青年鼠比较，去卵巢大鼠E2水平明显下降(P＜0.01)，核桃油干预组大鼠随核桃油剂量增加血清T-SOD 活性明显增加；MDA含量均显著降低，海马齿状回细胞Bcl-2表达增加，Bax表达下降。海马齿状回细胞凋亡减少。结论：野生山核桃油能减少去卵巢大鼠海马细胞的凋亡，对海马细胞有保护作用，其作用机制可能与提高机体的抗氧化能力、调节凋亡相关蛋白Bcl-2、Bax的表达有关。

关键词: 核桃油, 去卵巢大鼠, 海马, 凋亡

Abstract: Objective: To observe the effect of wild walnut oil on the antioxidant functions in variectomized rats and the apoptosis of hippocampal cells. Methods: Ovariectomized rats were used as elderly animal model and administrated intragastrically with wild walnut oil at various doses (2.4, 1.2, 0.6 g/(kg ·d)) for 9 days. The change of serum estradiol (E2) was determined by radioimmunoassay (RIA). The levels of T-SOD and MDA in hippocampus homogenates were determined by biochemical method. The expression of Bcl-2 and Bax in hippocampal dentate gyrus was evaluated by immunohistochemistry, and cell apoptosis in hippocampal dentate gyrus was observed by Hoechst staining. Results: Compared with young rats, the E2 level of ovariectomized rats revealed an obvious decrease (P ＜ 0.01), the T-SOD activity of wild walnut oil group exhibited a dose-dependant increase. Meanwhile, wild walnut oil could result in the reduction of MDA content, increased the expression of Bcl-2 and decreased the expression of Bax in hippocampal dentate gyrus cells. Cell apoptosis of hippocampal dentate gyrus was also inhibited. Conclusion: Wild walnut oil has protective effect against hippocampal cell apoptosis induced by ovariectomization. The mechanism of apoptosis inhibition may be related to the enhanced antioxidant activity and modulated expression of Bcl-2 and Bax.

Key words: walnut oil, ovariectomized rats, hippocamp, apoptosis

中图分类号:

TS255.1

陈默然，沈楠，雷钧涛，赵行宇. 野生山核桃油对去卵巢大鼠抗氧化能力与海马细胞凋亡的影响[J]. 食品科学, 2011, 32(9): 272-275.

CHEN Mo-ran，SHEN Nan，LEI Jun-tao， ZHAO Xing-yu. Effects of Wild Walnut Oil on Antioxidant Functions in Ovariectomized Rats and Apoptosis of Hippocampal Cells[J]. FOOD SCIENCE, 2011, 32(9): 272-275.

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野生山核桃油对去卵巢大鼠抗氧化能力与海马细胞凋亡的影响

Effects of Wild Walnut Oil on Antioxidant Functions in Ovariectomized Rats and Apoptosis of Hippocampal Cells

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