食品科学 ›› 2011, Vol. 32 ›› Issue (10): 79-83.doi: 10.7506/spkx1002-6630-201110018

• 工艺技术 • 上一篇    下一篇

甘肃河西沙区锁阳多糖提取工艺优化及含量测定

罗光宏,王 勤*,祖廷勋,陈天仁   

  1. 1.河西学院凯源生物技术开发中心,甘肃省微藻工程技术研究中心,甘肃省高校河西走廊特色资源利用省级重点实验室 2.甘肃省张掖市药品检验所
  • 出版日期:2011-05-25 发布日期:2011-04-08
  • 基金资助:
    甘肃省科技支撑计划项目(0804NKCG082);甘肃省教育厅研究生导师计划项目(0809-07); 甘肃省高校河西走廊特色资源利用省级重点实验室项目(XZ0707)

Optimum Extraction Process and Content Determination of Polysaccharides from Cynomorium songaricum Rupr. in Hexi Corridor Desert Area of Gansu

LUO Guang-hong1,WANG Qin2,*,ZU Ting-xun1,CHEN Tian-ren1   

  1. 1. The Provincial Key Laboratories of Characteristic Resource Utilization of Hexi Corridor in Gansu, Microalgae Engineering Research Center of Gansu Province, Kaiyuan Bio-tech Development Center, Hexi University, Zhangye 734000, China; 2. Zhangye Institute for Drug Control, Zhangye 734000, China
  • Online:2011-05-25 Published:2011-04-08

摘要: 采用水提醇沉法,利用L9(33)正交试验优选锁阳多糖的提取工艺,用硫酸苯酚比色法对甘肃河西沙区不同地区锁阳中多糖含量进行测定。结果表明:提取最佳工艺条件为料液比1:15(g/mL)、提取时间90min、提取次数为2次;甘肃河西沙区不同地区锁阳多糖含量差别显著。锁阳多糖可作为控制锁阳质量的一项指标性成分。

关键词: 锁阳, 多糖, 正交试验, 提取工艺, 含量测定

Abstract: The water extraction and ethanol precipitation of polysaccharides from the roots of Cynomorium songaricum Rupr.were optimized by orthogonal array design and the contents of polysaccharides in samples from different habitats in Hexi Corridor were determined by benzenol-H2SO4 method. Results indicated that the optimal extraction conditions were material/liquid ratio of 1:15 (g/mL), refluxing extraction duration of 90 min and repeated extraction number of 2. Root samples of Cynomorium songaricum Rupr. from different habitats in Hexi Corridor were significantly different from each other in their polysaccharide contents. Therefore, polysaccharide content can be used as a quality control indicator for Cynomorium songaricum Rupr..

Key words: Cynomorium songaricum Rupr., polysaccharide, orthogonal test, extraction process, content determination

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