食品科学 ›› 2011, Vol. 32 ›› Issue (15): 177-181.doi: 10.7506/spkx1002-6630-201115040

• 生物工程 • 上一篇    下一篇

柠檬苦素降解菌的分离筛选与分类鉴定

纪学芳,师俊玲*,张锦华   

  1. 西北农林科技大学食品科学与工程学院
  • 出版日期:2011-08-15 发布日期:2011-07-26
  • 基金资助:
    西北农林科技大学农业科技创新专项(CX200905)

Isolation and Identification of Limonin-degrading Strains Isolated from Fermented Grains during Vinegar Fermentation

JI Xue-fang,SHI Jun-ling*,ZHANG Jin-hua   

  1. (College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China)
  • Online:2011-08-15 Published:2011-07-26

摘要: 以新鲜醋醅为分离源,分离得到5株具有柠檬苦素降解活性的菌种,对柠檬苦素的最大降解率可达(58.86±2.81)%,发酵液的酶活力为(120.09±1.32)U/mL。基于形态特征、生理生化特性和16S rDNA序列分析结果,所得5 株菌均属芽孢杆菌属(Bacillus),其中2株为Bacillus siamensis,1株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),1株为短小芽孢杆菌(Bacillus pumilus),降解能力最强的1株为芽孢杆菌种Bacillus tequilensis。

关键词: 柑橘, 柠檬苦素, 降解, 分类鉴定, 芽孢杆菌

Abstract: Five strains with stronger limonin-degrading ability named as CS1, CS2, CS3, CS4 and CS5 were isolated from fresh fermented grains of vinegar. Among them, strain CS4 exhibited the highest limonin degradation rate of (58.86 ± 2.81)% and the highest enzyme activity of (120.09 ± 1.32) U/mL in fermentation broth. On the basis of their morphological, physiological and biochemical characteristics and 16S rDNA gene sequence, all of the 5 strains were identified as Bacillus genus, including 2 Bacillus siamensis strains, 1 Bacillus amyloliquefaciens strain, 1 Bacillus pumilus strain and 1 Bacillus tequilensis strain that had the highest limonin-degrading capability (strain CS4).

Key words: citrus, limonin, degradation, Bacillus

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