食品科学 ›› 2011, Vol. 32 ›› Issue (16): 76-79.doi: 10.7506/spkx1002-6630-201116016

• 工艺技术 • 上一篇    下一篇

超声提取鼠尾草叶多糖工艺优化及DPPH自由基清除能力评价

祝元婷,吴文林,张 利*,刘 琪   

  1. 四川农业大学生命科学与理学院
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    四川省青年科技基金资助项目(08ZQ026-034);四川省科技厅科技支撑计划资助项目(2008FZ0148;2008SZ0074)

Optimization of Ultrasonic Extraction of Polysaccharides from Salvia officinalis L. Leaves and Their DPPH Free Radical Scavenging Activity

ZHU Yuan-ting,WU Wen-lin,ZHANG Li*,LIU Qi   

  1. (College of Biology and Science, Sichuan Agricultural University, Ya'an 625014, China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 以鼠尾草(Salvia officinalis L.)叶为材料,采用单因素试验和正交试验,探讨超声提取其多糖的最佳工艺条件,并利用1,1-二苯基苦基苯肼(DPPH)自由基清除法评价该多糖的自由基清除能力。结果表明:超声提取多糖的最佳工艺条件为:提取时间7min、提取2次、料液比1:40,在此条件下鼠尾草叶多糖的提取率为3.27%。同时该多糖对DPPH自由基有较好的清除作用,当质量浓度为160μg/mL时,清除率达到82%,并且对DPPH自由基清除能力IC50为112μg/mL。

关键词: 鼠尾草, 多糖, 超声提取, 1, 1-二苯基苦基苯肼(DPPH)

Abstract: The conditions for ultrasonic extraction of polysaccharides from Salvia officinalis L. leaves were optimized by one-factor-at-a-time and orthogonal array design methods. A material-to-liquid ratio of 1:40 for 7 min extractions performed twice proved optimal. The resultant polysaccharide yield was 3.27%. The polysaccharide extract obtained had excellent DPPH free radical scavenging effect, revealing a scavenging rate of 82% at the concentration of 160μg/mL and an IC50 of 112μg/mL.

Key words: Salvia officinalis, polysaccharide, ultrasonic extraction, 1,1-diphenyl-2-picrylhydrazyl (DPPH)

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