食品科学 ›› 2011, Vol. 32 ›› Issue (16): 71-75.doi: 10.7506/spkx1002-6630-201116015

• 工艺技术 • 上一篇    下一篇

湘西节节草总黄酮的超声波提取及抗氧化研究

张俊生,陈莉华*,张文龙   

  1. 吉首大学化学化工学院
  • 出版日期:2011-08-25 发布日期:2011-07-26
  • 基金资助:
    科技部科技型中小企业技术创新基金资助项目(10C26214302421;11C26414305373); 吉首大学校级科研计划项目(11JDY051)

Ultrasonic-assisted Ethanol Extraction and Antioxidant Activity of Total Flavonoids from Equisetum ramosissimum Desf. Grown in West Hunan

ZHANG Jun-sheng,CHEN Li-hua*,ZHANG Wen-long   

  1. (College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China)
  • Online:2011-08-25 Published:2011-07-26

摘要: 以湘西蕨类植物节节草为原料,采用超声波辅助法提取总黄酮。通过单因素及正交试验优化提取工艺条件,考察总黄酮对羟自由基的清除效果及在不同时间、温度下对油脂的抗氧化性能。结果表明,当在乙醇体积分数45%、料液比1:30 (g/mL)、超声提取时间30min、超声温度60℃最佳条件下,节节草中总黄酮得率为1.16%。该黄酮提取物对羟自由基清除效果随浓度的增大而升高,对茶籽油的抗氧化效果较动物油脂好。

关键词: 节节草, 黄酮, 提取, 油脂, 抗氧化性

Abstract: One-factor-at-a-time and orthogonal array design methods were used to optimize the conditions for ultrasonic-assisted ethanol extraction of total flavonoids from the dried shoot of Equisetum ramosissimum Desf. grown in west Hunan province, China. The extraction yield of total flavonoids was 1.16% under the optimal extraction conditions: 45% ethanol aqueous solution as the extraction solvent, material-to-liquid ratio 1:30 (g/mL), ultrasonic treatment time 30 min, and extraction temperature 60 ℃. The total flavonoids could scavenge hydroxyl free radicals in a concentration-dependent fashion and had stronger inhibitory effect against lipid peroxidation in tea seed oil than in lard.

Key words: Equisetum ramosissimum Desf., total flavonoids, extraction, fat and oil, antioxidation

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