食品科学 ›› 2011, Vol. 32 ›› Issue (17): 147-150.doi: 10.7506/spkx1002-6630-201117029

• 基础研究 • 上一篇    下一篇

海蜇糖蛋白分离纯化及免疫活性研究

任国艳,梁旺春,詹永献,郭金英,樊金玲,陈树兴   

  1. 河南科技大学食品与生物工程学院
  • 发布日期:2011-08-30
  • 基金资助:
    国家自然科学基金项目(31000765);河南科技大学青年基金项目(2010QN003)

Purification and Immune Activity of Glycoprotein Isolated from Jellyfish Oral Arms

REN Guo-yan,LIANG Wang-chun,ZHAN Yong-xian,GUO Jin-ying,FAN Jin-ling,CHEN Shu-xing   

  1. (College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China)
  • Published:2011-08-30

摘要: 以海蜇头为原料,通过乙醇分级沉淀、柱层析等分离纯化方法,获得3种组分均一的糖蛋白JGP-Ⅱ、JGP-Ⅲ、JGP-Ⅳ。检测3种糖蛋白对小鼠脾淋巴细胞增殖作用和转化能力的影响,研究其免疫活性。结果表明:3种糖蛋白对小鼠脾淋巴细胞均有增殖作用;对脾淋巴细胞转化能力具有促进作用。

关键词: 海蜇, 糖蛋白, 分离纯化, 免疫活性

Abstract: Three kinds of homogeneous glycoprotein (JGP-II, JGP-III and JGP-IV) were isolated from jellyfish oral arms, and their immune activity was studied. The results showed that the three kinds of glycoprotein could improve the proliferation and transformation of mouse spleen lymphocytes.

Key words: jellyfish oral arms, glycoprotein, isolation and purification, immune activity

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