食品科学 ›› 2012, Vol. 33 ›› Issue (8): 296-300.doi: 10.7506/spkx1002-6630-201208065

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系统Ⅱ乙烯对采后番茄果实抗冷过程中抗氧化酶的影响

吕胜男1,赵瑞瑞1,申 琳1,赵丹莹3,生吉萍1,2,*   

  1. 1.中国农业大学食品科学与营养工程学院 2.中国人民大学农业与农村发展学院 3.北京市西城区疾病预防控制中心
  • 出版日期:2012-04-25 发布日期:2012-03-31
  • 基金资助:
    国家自然科学基金面上项目(31071623;30972065);国家公益性行业(农业)科研专项(200803033)

Effect of System-2 Ethylene on Antioxidant Enzyme Activities in Cold Resistance Process of Tomato Fruits

LU . . Sheng-nan1,ZHAO Rui-rui1,SHEN Lin1,ZHAO Dan-ying3,SHENG Ji-ping1,2,*   

  1. (1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. School of Agricultural Economics and Rural Development, Renmin University of China, Beijing 100872, China; 3. Beijing Xicheng District Center for Disease Control and Prevention, Beijing 100011, China)
  • Online:2012-04-25 Published:2012-03-31

摘要: 以反义ACS2 番茄、野生型丽春果实和经乙烯利处理的反义ACS2 番茄果实分别作为系统Ⅱ乙烯缺失模型、系统Ⅱ乙烯正常合成模型和系统Ⅱ乙烯补偿模型,研究系统Ⅱ乙烯对低温胁迫下氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的影响。研究发现,丽春果实和经乙烯利处理的反义ACS2果实较未处理的反义ACS2果实组冷害指数低,电解质渗透率的增长被抑制,CAT、APX、SOD平均活性水平提高,而两组的POD活性增长分别被抑制和诱导。这说明系统Ⅱ乙烯能提高CAT、APX、SOD活性水平并调控POD活性变化,对增强低温胁迫下番茄果实的抗冷能力有积极作用。

关键词: 乙烯, 抗氧化酶, 氧化胁迫, 抗冷性, 番茄

Abstract: To analyze the effect of system-2 ethylene on the activities of catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and superoxide dismutase (SOD) during exposure to cold stress, anti-sense ACS2, wild-type Lichun and ethephon-treated anti-sense ACS2 tomato fruits were used to the models of non-system-2 ethylene synthesis, normal system-2 ethylene synthesis and compensative system-2 ethylene synthesis, respectively. It was indicated that wild-type Lichun and ethephon-treated anti-sense ACS2 tomato fruits decreased chilling injury index, inhibited the increase of electrolyte leakage and increased the average activities of CAT, APX and SOD compared with native anti-sense ACS2 tomato fruits. The increase of POD activity was inhibited in wild-type Lichun but induced in ethephon-treated anti-sense ACS2. From these results, it can be concluded that system-2 ethylene can improve the cold resistance of tomato during exposure to cold stress by increasing CAT, APX and SOD activities and regulating POD activity.

Key words: ethylene, antioxidant enzymes, oxidative stress, cold resistance, tomato

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