食品科学 ›› 2013, Vol. 34 ›› Issue (10): 6-10.doi: 10.7506/spkx1002-6630-201310002

• 工艺技术 • 上一篇    下一篇

正交试验优化马铃薯龙葵素提取技术

钟 源1,肖文军1,2,*,马 蕊1,李政泽1   

  1. 1.湖南农业大学园艺园林学院,湖南 长沙 410128; 2.湖南农业大学 国家植物功能成分利用工程技术研究中心,湖南 长沙 410128
  • 收稿日期:2012-03-01 修回日期:2013-03-13 出版日期:2013-05-25 发布日期:2013-05-07
  • 通讯作者: 肖文军 E-mail:xiaowenjun88@sina.com;xiaowenjungong@163.com
  • 基金资助:

    “十二五”国家支撑计划资助项目

Optimization of Extraction of Solanine from Potato by Orthogonal Array Design

ZHONG Yuan1,XIAO Wen-jun1,2,*,MA Rui1,LI Zheng-ze1   

  1. 1. College of Horticultural and Landscape, Hunan Agricultural University, Changsha 410128, China;
    2. National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients,
    Hunan Agricultural University, Changsha 410128, China
  • Received:2012-03-01 Revised:2013-03-13 Online:2013-05-25 Published:2013-05-07

摘要:

以变绿、发芽的马铃薯皮为原料,在系统优化筛选双溶剂提取法、超声波辅助提取法提取马铃薯龙葵素的基础上,比较乙醇回流提取、微波辅助提取、双溶剂提取、超声波辅助提取4种方法对马铃薯龙葵素的提取效果。结果表明,双溶剂提取马铃薯龙葵素的最佳工艺为提取温度70℃、提取时间17h、料液比1:20、乙醇乙酸比10:2、原料粒径0.3mm,龙葵素得率为0.6417%,4个因素对马铃薯龙葵素提取效果的影响大小为乙醇乙酸比>粒径>料液比>时间;超声波辅助提取的最佳工艺为70%乙醇、pH3、超声温度65℃、超声时间为20min、料液比为1:15、原料粒径0.3mm、超声2次,龙葵素得率达到0.8356%,4个因素对马铃薯龙葵素提取效果的影响大小为粒径>料液比>超声温度>超声时间;微波辅助提取和乙醇回流的提取得率分别为0.5489%、0.1881%。比较4种提取方法,超声波辅助提取时间短,溶剂用量少,提取效果最好。

关键词: 马铃薯龙葵素, 超声波辅助提取, 微波辅助提取, 双溶剂提取, 乙醇回流提取

Abstract:

Abstract:Two different methods to extract solanine from sprouted potatoes, dual-solvent extraction and ultrasonicassisted
extraction, were optimized in this study. Moreover, the two extraction methods were compared with ethanol reflux
extraction and microwave-assisted extraction. The results showed that the optimum extraction conditions for dual-solvent
extraction were water-bath temperature 70 ℃, 17 h , ratio of material to liquid of 1:20, ratio of ethanol to acetic acid of 10:2
and material particle size of 0.3 mm. Under these conditions, the extraction yield of solanine was 0.6417%, and the influence
of factors on the yield of solanine decreased in the following order: ratio of ethanol to acetic acid > particle size > ratio of
material to liquid > time. The optimal extraction conditions for ultrasonic-assisted extraction were ethanol concentration of
70%, pH 3, water-bath temperature of 65 ℃, ultrasonic time 20 min, ratio of material to liquid of 1:15, particle size 0.3 mm
and two extraction cycles, in the decreasing order of importance: particle size > ratio of material to liquid > water-bath
temperature > ultrasonic time. Under these conditions, the extraction yield of solanine was 0.8356% compared to 0.5489%
with microwave-assisted extraction and 0.1881% with ethanol reflux extraction. In conclusion, ultrasonic-assisted extraction
is a time-saving, solvent-saving and highly effective method.

Key words: potato, solanine, ultrasonic-assisted extraction, microwave-assisted extraction, dual-solvent extraction, ethanol reflux extraction

中图分类号: