食品科学 ›› 2013, Vol. 34 ›› Issue (10): 103-106.doi: 10.7506/spkx1002-6630-201310022

• 工艺技术 • 上一篇    下一篇

正交试验优化鼠曲草色素提取及其稳定性

丁 月,徐 超,陈霞霞,郑易林,万力婷,陈伊梦,王鸿飞   

  1. 宁波大学食品科学与工程系,浙江 宁波 315211
  • 收稿日期:2012-03-09 修回日期:2013-03-19 出版日期:2013-05-25 发布日期:2013-05-07
  • 通讯作者: 王鸿飞 E-mail:wanghongfei@nbu.edu.cn
  • 基金资助:

    宁波大学SRIP项目

Extraction Optimization by Orthogonal Array Design and Stability of Pigment from Herba Gnaphaii Affinis

DING Yue,XU Chao,CHEN Xia-xia,ZHENG Yi-lin,WAN Li-ting,CHEN Yi-meng,WANG Hong-fei   

  1. Department of Food Science and Engineering, Ningbo University, Ningbo 315211, China
  • Received:2012-03-09 Revised:2013-03-19 Online:2013-05-25 Published:2013-05-07

摘要:

以鼠曲草为试验原料,采用浸提法提取鼠曲草色素,分别研究乙醇体积分数、料液比及浸提时间等因素对提取效果的影响,在单因素试验的基础上,通过L9(34)正交试验优化提取参数。同时考察温度、光照、溶液pH值及金属离子对鼠曲草色素稳定性的影响。结果表明,鼠曲草色素最优提取工艺为乙醇体积分数90%、料液比1:300(g/mL),浸提时间100min。鼠曲草色素的最大吸收波长为416nm,对光照和温度稳定性较好,在碱性条件下色素比较稳定,Cu2+、Ca2+及Zn2+对色素稳定性影响较大。

关键词: 鼠曲草, 色素, 正交试验, 稳定性

Abstract:

A solvent extraction technique was developed to extract pigment from Herba Gnaphaii Affinis and optimized by
investigating the effects of ethanol concentration, solid-to-liquid ratio and extraction time on extraction efficiency using onefactor-
at-a-time and orthogonal array design. The stability of the pigment obtained was also investigated to temperature, light,
pH and metal ions. The optimal extraction conditions were found to be 90% ethanol, a solid-to-liquid ratio of 1:300 (g/mL)
and 100 min. The pigment obtained under these conditions had maximum absorption at 416 nm and was stable to light,
temperature and alkaline condition but greatly influenced by Cu2+, Ca2+ and Zn2+.

Key words: Herba Gnaphaii Affinis, pigment, orthogonal dsign, stability

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