食品科学 ›› 2013, Vol. 34 ›› Issue (13): 152-156.doi: 10.7506/spkx1002-6630-201313033

• 生物工程 • 上一篇    下一篇

气调保鲜冷却猪肉中假单胞菌最小检出生长时间模型的构建

邱 静,董庆利*,胡孟晗   

  1. 上海理工大学医疗器械与食品学院,上海 200093
  • 收稿日期:2012-03-05 修回日期:2013-05-10 出版日期:2013-07-15 发布日期:2013-06-28
  • 通讯作者: 董庆利 E-mail:dongqingli@126.com
  • 基金资助:

    国家自然科学基金面上项目(31271896);上海市自然科学基金面上项目(12ZR1420500)

Establishment of Time-to-detection Model of Pseudomonas spp. in Atmosphere Modified Packaged Chilled Pork

QIU Jing,DONG Qing-li*,HU Meng-han   

  1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
  • Received:2012-03-05 Revised:2013-05-10 Online:2013-07-15 Published:2013-06-28
  • Contact: DONG Qing-li E-mail:dongqingli@126.com

摘要:

主要将气调保鲜冷却猪肉中的优势腐败菌之一的假单胞菌接种到灭菌肉上,在(4±1)℃条件下,通过运 用线性回归方程构建不同气调比例下假单胞菌的最小检出生长时间(time-to-detection,TTD)模型。结果表明:假单 胞菌的最小检出生长时间在无CO2的情况下,会随着O2体积分数的增加而减小;但会随着CO2体积分数的增加而增 大。初始接种量的增大也会减小最小检出生长时间,但是当接种浓度大于102CFU/mL,减小并不明显。TTD模型的 拟合度良好,回归系数(r2)高达0.959,实际观测值与模型预测值的判定系数R2均高于0.986,模型的偏差因子(Bf)和 准确因子(Af),分别为1.012和1.086,均接近1。

关键词: 气调保鲜, 冷却猪肉, 最小检出生长时间, TTD模型, 假单胞菌

Abstract:

This study was to establish a growth probability model of Pseudomonas spp. in atmosphere modified
packaged chilled pork at (4 ± 1) ℃. Linear regression was used to establish TTD (time-to-detection) models of
Pseudomonas spp. in different atmosphere conditions. Disinfected pock was inoculated with Pseudomonas spp., a specific
spoilage organisms in chilled pock. The results showed that in the absence of CO2, the TTD decreased with increasing
O2 concentration, but increased as CO2 concentration increased. TTD reduced sharply as initial inoculation increased.
However, no apparent decrease in TTD was found when initial inoculation was over 102 CFU/mL. The TTD models
developed in this study showed a regression coefficient (r2) higher than 0.959 and the coefficients of determination (R2)
for the observed versus predicted data were higher than 0.986. In addition, the Bf and Af for the TTD models were 1.012
and 1.086, respectively. Both were close to 1.

Key words: modified atmosphere package, chilled pork, time-to-detection, TTD model, Pseudomonas spp.

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