食品科学 ›› 2019, Vol. 40 ›› Issue (11): 7-13.doi: 10.7506/spkx1002-6630-20180510-163

• 基础研究 • 上一篇    下一篇

富马酸钠对荧光假单胞菌群体感应现象及其腐败活性的抑制作用

孙晓佳1,李婷婷2,*,赫彬彬1,梅永超1,王当丰1,谢 晶3,励建荣1,*   

  1. 1.渤海大学食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁省食品安全重点实验室,辽宁省高等学校生鲜食品产业技术研究院,辽宁 锦州 121013;2.大连民族大学生命科学学院,辽宁 大连 116600;3.上海海洋大学食品学院,上海 201306
  • 出版日期:2019-06-15 发布日期:2019-06-28
  • 基金资助:
    国家自然科学基金面上项目(31471639);国家自然科学基金青年科学基金项目(31301572);“十三五”国家重点研发计划重点专项(2017YFD0400106;2018YFD0400600)

Inhibitory Effect of Sodium Fumarate on Quorum Sensing and Spoilage Capacity of Pseudomonas fluorescens

SUN Xiaojia1, LI Tingting2,*, HE Binbin1, MEI Yongchao1, WANG Dangfeng1, XIE Jing3, LI Jianrong1,*   

  1. 1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Laboratory of Liaoning Province, the Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, College of Food Science and Technology, Bohai University, Jinzhou 121013, China; 2. College of Life Science, Dalian Minzu University, Dalian 116600, China; 3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2019-06-15 Published:2019-06-28

摘要: 以报告菌株紫色杆菌CV026为检测模型,探究富马酸钠对荧光假单胞菌群体感应现象及其腐败特性的影响。采用酶标法及光学显微镜观察富马酸钠对荧光假单胞菌生物被膜的抑制效果,利用气相色谱-质谱分析富马酸钠对荧光假单胞菌分泌信号分子的影响。结果表明:在亚抑菌浓度下,富马酸钠可以显著降低紫色杆菌CV026紫色菌素的产生量(P<0.05);当质量浓度为2.0 mg/mL时,富马酸钠对紫色菌素的抑制率达50.95%,且不影响CV026菌株的生长。富马酸钠能有效抑制荧光假单胞菌生物被膜形成、群集与泳动、胞外蛋白酶活性等相关腐败特性,且质量浓度与其抑制作用呈正相关。另外,由气相色谱-质谱检测结果可知,富马酸钠对荧光假单胞菌N-酰基高丝氨酸内酯信号分子的分泌有较好的抑制效果。因此,富马酸钠具有较好的群体感应抑制活性,可作为新型群体感应抑制剂用于水产品贮藏保鲜。

关键词: 富马酸钠, 荧光假单胞菌, 群体感应, 腐败活性, 抑制作用

Abstract: In this paper, Chromobacterium violaceum CV026 strain was used as a test model to investigate the effect of sodium fumarate on quorum sensing and spoilage capacity of Pseudomonas fluorescens. The inhibitory effect of sodium fumarate on biofilm formation was evaluated with a microplate reader and optical microscopy, and its effect on the secretion of signal molecules in Pseudomonas fluorescens was determined by gas chromatography-mass spectrometry (GC-MS). The results showed that sodium fumarate could significantly reduce the production of violacein in strain CV026 at sub-minimum inhibitory concentrations. Violacein production was inhibited 50.95% by sodium fumarate at 2.0 mg/mL while not affecting the growth of CV026. Sodium fumarate could effectively inhibit spoilage characteristics of Pseudomonas fluorescens as reflected by biofilm formation, swarming and swimming motility, and extracellular protease activity and this effect was positively correlated with its concentration. By GC-MS analysis, we also found that sodium fumarate significantly inhibited the secretion of the signal molecules N-acylhomoserine lactones (AHLs). Therefore, sodium fumarate has great inhibitory activity and can be developed as a quorum sensing inhibitor for refrigerated storage and preservation of aquatic products.

Key words: sodium fumarate, Pseudomonas fluorescens, quorum sensing, spoilage capacity, inhibitory effects

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