食品科学

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单核细胞增生李斯特氏菌拮抗菌的分离鉴定及其抑菌活性

许红岩1,段效辉1,*,李小清2   

  1. 1.烟台出入境检验检疫局,山东 烟台 264000;2.临沂市技术监督信息所,山东 临沂 276000
  • 出版日期:2013-08-15 发布日期:2013-09-03
  • 通讯作者: 段效辉
  • 基金资助:

    国家质检总局科研项目(2005IK022)

Isolation and Identification of Antagonistic Strain against Listeria monocytogenes

XU Hong-yan1,DUAN Xiao-hui1,*,LI Xiao-qing2   

  1. 1. Yantai Entry-exit Inspection and Quaratine Bureau, Yantai 264000, China;
    2. Linyi City Technical Supervision Information Institute, Linyi 276000, China  
  • Online:2013-08-15 Published:2013-09-03
  • Contact: DUAN Xiao-hui

摘要:

从烟台近郊土壤中分离出一株单核细胞增生李斯特氏菌拮抗微生物sly-3,利用传统生理生化和分子生物学相结合的方法对其进行鉴定,并采用生物测定的方法评价其抑菌活性。菌株的形态学特征和生理生化特征与枯草芽孢杆菌基本一致,其16S rDNA序列与GenBank中已鉴定的枯草芽孢杆菌的16S rDNA序列同源性达99%以上,据此初步确定其为枯草芽孢杆菌。抑菌谱研究发现,该菌仅对革兰氏阳性菌有抑制作用,而对革兰氏阴性菌无明显抑制作用。sly-3菌株发酵过滤液初步作用于水产品中单核细胞增生李斯特氏菌,发现可有效抑制该致病菌的生长,且在高温处理条件下保留较高的抑菌活性,在食品保鲜中显示出了良好的应用前景。

关键词: 水产品, 单核细胞增生李斯特氏菌, 拮抗菌

Abstract:

In this study, sly-3 strain that can inhibit Listeria monocytogenes was isolated from soil in Yantai suburb. The
morphological, physiological and biochemical characteristics of the isolated strain were basically consistent with those of
Bacillus subtilis and the homology of 16S rDNA sequence between sly-3 and B. subtilis CICC10034 was 99%. On the basis of
these results, sly-3 was finally identified as Bacillus subtilis. The antagonistic activity of sly-3 strain also suggested that only G+
bacteria could be inhibited but G- bacteria could not be inhibited. Meanwhile, the 24 h culture filtrate of sly-3 exhibited high
inhibitory activity against Listeria monocytogenes from export aquatic products.

Key words: aquatic products, Listeria monocytogenes, antagonistic strain

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