食品科学

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嗜酸乳杆菌在牛羊乳基质中发酵性能差异比较研究

葛武鹏1,王秀玲1,赵 蕊1,陈 瑛2,杨 静3,秦立虎4   

  1. 1.西北农林科技大学食品科学与工程学院,陕西 杨凌 712100;2.陕西省质量技术监督局信息中心,陕西 西安 710006;
    3.陕西杨凌质量技术检测检验所,陕西 杨凌 712100;4.西安市奶牛育种中心,陕西 西安 710065
  • 出版日期:2013-08-15 发布日期:2013-09-03
  • 通讯作者: 葛武鹏
  • 基金资助:

    西北农林科技大学人才基金项目(Z111020923)

Comparison of Fermentation Properties of Lactobacillus acidophilus in Cow and Goat Milk

GE Wu-peng1,WANG Xiu-ling1,ZHAO Rui1,CHEN Ying2,YANG Jing3,QIN Li-hu4   

  1. 1. College of Food Science and Technology, Northwest A&F University, Yangling 712100, China;2. Information Center of
    Shaanxi Bureau of Quality and Technical Supervision, Xi’an 710006, China;3. Institution of Inspection of Yangling Bureau of
    Quality and Technical Supervision, Yangling 712100, China;4. Center of Xi’an Dairy Cattle Breeding, Xi’an 710065, China
  • Online:2013-08-15 Published:2013-09-03
  • Contact: GE Wu-peng

摘要:

探讨嗜酸乳杆菌在牛、羊乳基质中的增菌发酵规律及产酸、产香性能,比较其差异性。结果表明:以羊乳为基质37℃发酵14h活菌数最高可达5.37×1010CFU/mL,pH值降至4.15,滴定酸度120.00ºT,丁二酮生成量为18.25mg/L,乙醛生成量为11.23mg/L。以牛乳为基质相同条件活菌数可达9.33×109 CFU/mL,pH值降至4.17,滴定酸度110.90 ºT,丁二酮生成量为17.77mg/L,乙醛生成量为9.85mg/L,嗜酸乳杆菌以牛、羊乳为基质37℃发酵14h,发酵增菌效率及产乙醛能力差异极显著(P<0.01),滴定酸度变化差异显著(P<0.05),而pH值和产丁二酮能力差异不显著(P>0.05)。说明嗜酸乳杆菌在羊乳基质中主要发酵性能优于牛乳。

关键词: 嗜酸乳杆菌, 牛乳, 羊乳, 发酵性能

Abstract:

Fermentation properties such as acid- or flavor-producing capability and proliferation pattern were determined
for Lactobacillus acidophilus in cow milk in comparison to cells in goat milk aiming to provide a useful guideline to develop
probiotic fermented milk and functional dairy products. The results showed that the maximum viable cell population of
Lactobacillus acidophilus (5.37 × 1010 CFU/mL) was obtained after 14 h of fermentation at 37 ℃ in goat milk and moreover, the
pH was reduced to 4.15, providing a titratable acidity of 120.00 ºT, and 18.25 mg/L of diacetyl and 11.23 mg/L of acetaldehyde
were produced. In contrast, the maximum viable cell population of Lactobacillus acidophilus was 9.33 × 109 CFU/mL
upon using cow milk instead of goat milk for fermentation under identical conditions and the pH was reduced to 4.17,
resulting in a titratable acidity of 110.90 ºT, along with the production of 17.77 mg/L of diacetyl and 9.85 mg/L of
acetaldehyde. No significant difference in diacetyl production or pH was observed for Lactobacillus acidophilus after 14 h
of fermentation in cow and goat milk (P > 0.05), while there were a highly significant difference (P < 0.01) in bacterial
proliferation and acetaldehyde production and a significant difference (P < 0.05) in titratable acidity. Conclusion: Compared
with cow milk, Lactobacillus acidophilus has better fermentation performance in goat milk.

Key words: Lactobacillus acidophilus, cow milk, goat milk, fermentation properties

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